In vitro preliminary study of osteoblast response to surface roughness of titanium discs and topical application of melatonin

To observe human osteoblast behavior cultured in vitro on titanium discs (Ti) in relation to surface roughness and melatonin application. Human osteoblasts (MG-63) were cultured on 60 Ti6Al4V discs divided into three groups: Group I: discs treated with dual acid etching; Group II dual acid etching a...

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Published in:Medicina oral, patología oral y cirugía bucal patología oral y cirugía bucal, 2015-01, Vol.20 (1), p.e88-e93
Main Authors: Solá-Ruiz, Maria-Fernanda, Pérez-Martínez, Carolina, Martín-del-Llano, José-Javier, Carda-Batalla, Carmen, Labaig-Rueda, Carlos
Format: Article
Language:English
Subjects:
Administration, Topical
Cells, Cultured
Dental Implants
Dentistry
Humans
Melatonin - administration & dosage
Melatonin - pharmacology
Osteoblasts - drug effects
Osteoblasts - physiology
Surface Properties
Titanium
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Summary:To observe human osteoblast behavior cultured in vitro on titanium discs (Ti) in relation to surface roughness and melatonin application. Human osteoblasts (MG-63) were cultured on 60 Ti6Al4V discs divided into three groups: Group I: discs treated with dual acid etching; Group II dual acid etching and blasting with calcium phosphate particles; Group III (control) machined discs. Surface roughness and topography of the discs were examined with scanning electron microscope (SEM) and confocal laser scanning electron microscope( CLSM). Osteoblast adhesion, proliferation and cell morphology were determined by means of fluorescence microscopy with Image-Pro Plus software and SEM. Group II presented the roughest discs, while the least rough were Group III. Cell adhesion was greatest in Group II. The addition of melatonin improved cell proliferation. 1. Surface treatments (dual acid etching, calcium phosphate impaction) increase surface roughness in comparison with machined titanium. 2. Greater surface roughness tends to favor cell adhesion after 24-hour cell culture. 3. The addition of melatonin tends to favor osteoblast proliferation.
ISSN:1698-6946
1698-4447
1698-6946
DOI:10.4317/medoral.19953