Overlapping local and long-range RNA-RNA interactions modulate dengue virus genome cyclization and replication

The dengue virus genome is a dynamic molecule that adopts different conformations in the infected cell. Here, using RNA folding predictions, chemical probing analysis, RNA binding assays, and functional studies, we identified new cis-acting elements present in the capsid coding sequence that facilit...

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Bibliographic Details
Published in:Journal of virology 2015-03, Vol.89 (6), p.3430-3437
Main Authors: de Borba, Luana, Villordo, Sergio M, Iglesias, Nestor G, Filomatori, Claudia V, Gebhard, Leopoldo G, Gamarnik, Andrea V
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Animals
Base Sequence
Capsid Proteins - chemistry
Capsid Proteins - genetics
Capsid Proteins - metabolism
Culicidae - virology
Dengue virus
Dengue Virus - chemistry
Dengue Virus - genetics
Dengue Virus - metabolism
DNA Replication
Gene Expression Regulation, Viral
Genome and Regulation of Viral Gene Expression
Genome, Viral
Molecular Sequence Data
Nucleic Acid Conformation
Regulatory Elements, Transcriptional
RNA, Viral - chemistry
RNA, Viral - genetics
RNA, Viral - metabolism
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Summary:The dengue virus genome is a dynamic molecule that adopts different conformations in the infected cell. Here, using RNA folding predictions, chemical probing analysis, RNA binding assays, and functional studies, we identified new cis-acting elements present in the capsid coding sequence that facilitate cyclization of the viral RNA by hybridization with a sequence involved in a local dumbbell structure at the viral 3' untranslated region (UTR). The identified interaction differentially enhances viral replication in mosquito and mammalian cells.
ISSN:0022-538X
1098-5514
DOI:10.1128/JVI.02677-14