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Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains
Abstract Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variet...
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| Published in: | G3 : genes - genomes - genetics 2015-03, Vol.5 (3), p.361-370 |
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| Main Authors: | , , , , , , , , , , , , , , , , |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c556t-51f3ae198ae2870cda86b1058abf0431c49f3e04b53ee5552a3c7ba35da75cd73 |
| container_end_page | 370 |
| container_issue | 3 |
| container_start_page | 361 |
| container_title | G3 : genes - genomes - genetics |
| container_volume | 5 |
| creator | Chen, Jun-Song Beckley, Janel R McDonald, Nathan A Ren, Liping Mangione, MariaSanta Jang, Sylvia J Elmore, Zachary C Rachfall, Nicole Feoktistova, Anna Jones, Christine M Willet, Alaina H Guillen, Rodrigo Bitton, Danny A Bähler, Jürg Jensen, Michael A Rhind, Nick Gould, Kathleen L |
| description | Abstract
Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of different stress conditions. We identified new genes involved in DNA metabolism, completion of the cell cycle, and morphogenesis. This subset of nonessential gene deletions will add to the toolkits available for the study of biological processes in S. pombe. |
| doi_str_mv | 10.1534/g3.114.015701 |
| format | article |
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Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of different stress conditions. We identified new genes involved in DNA metabolism, completion of the cell cycle, and morphogenesis. This subset of nonessential gene deletions will add to the toolkits available for the study of biological processes in S. pombe.</description><identifier>ISSN: 2160-1836</identifier><identifier>EISSN: 2160-1836</identifier><identifier>DOI: 10.1534/g3.114.015701</identifier><identifier>PMID: 25552606</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Cell Division - genetics ; DNA Damage - genetics ; Gene Deletion ; Genes, Fungal ; Investigations ; Schizosaccharomyces - cytology ; Schizosaccharomyces - genetics ; Schizosaccharomyces - metabolism</subject><ispartof>G3 : genes - genomes - genetics, 2015-03, Vol.5 (3), p.361-370</ispartof><rights>2015 Chen et al. 2015</rights><rights>Copyright © 2015 Chen et al.</rights><rights>Copyright © 2015 Chen 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c556t-51f3ae198ae2870cda86b1058abf0431c49f3e04b53ee5552a3c7ba35da75cd73</citedby><cites>FETCH-LOGICAL-c556t-51f3ae198ae2870cda86b1058abf0431c49f3e04b53ee5552a3c7ba35da75cd73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349090/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349090/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25552606$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Jun-Song</creatorcontrib><creatorcontrib>Beckley, Janel R</creatorcontrib><creatorcontrib>McDonald, Nathan A</creatorcontrib><creatorcontrib>Ren, Liping</creatorcontrib><creatorcontrib>Mangione, MariaSanta</creatorcontrib><creatorcontrib>Jang, Sylvia J</creatorcontrib><creatorcontrib>Elmore, Zachary C</creatorcontrib><creatorcontrib>Rachfall, Nicole</creatorcontrib><creatorcontrib>Feoktistova, Anna</creatorcontrib><creatorcontrib>Jones, Christine M</creatorcontrib><creatorcontrib>Willet, Alaina H</creatorcontrib><creatorcontrib>Guillen, Rodrigo</creatorcontrib><creatorcontrib>Bitton, Danny A</creatorcontrib><creatorcontrib>Bähler, Jürg</creatorcontrib><creatorcontrib>Jensen, Michael A</creatorcontrib><creatorcontrib>Rhind, Nick</creatorcontrib><creatorcontrib>Gould, Kathleen L</creatorcontrib><title>Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains</title><title>G3 : genes - genomes - genetics</title><addtitle>G3 (Bethesda)</addtitle><description>Abstract
Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of different stress conditions. We identified new genes involved in DNA metabolism, completion of the cell cycle, and morphogenesis. This subset of nonessential gene deletions will add to the toolkits available for the study of biological processes in S. pombe.</description><subject>Cell Division - genetics</subject><subject>DNA Damage - genetics</subject><subject>Gene Deletion</subject><subject>Genes, Fungal</subject><subject>Investigations</subject><subject>Schizosaccharomyces - cytology</subject><subject>Schizosaccharomyces - genetics</subject><subject>Schizosaccharomyces - metabolism</subject><issn>2160-1836</issn><issn>2160-1836</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqFkU1LxDAQhoMoKurRq-Towa7Jpmm7F0F2_QK_8OMcpum0jbRNSbrK-k_8t0ZXRU_mksA8eYaZl5BdzkZciviwEiPO4xHjMmV8hWyOecIinolk9dd7g-x4_8TCkTJJ4mSdbIyllOOEJZvk7aLAbjCl0TAY21Fb0mt8obcNLNB5ajo6xaahM_NsfKgf0Nn1MZ1BCxXSO_S97TweUOgKemVdX9sKO_TG04fa2XlV02kABjfX3_J7XZtX60HrGpxtFxo97W2bI51hg5_U_eDAdH6brJXQeNz5urfI4-nJw_Q8urw5u5geX0Y6jDNEkpcCkE8ywHGWMl1AluScyQzyksWC63hSCmRxLgXix9ggdJqDkAWkUhep2CJHS28_z1ssdFiHg0b1zrTgFsqCUX8rnalVZZ9VLOIJm7AgiJYC7az3Dsufv5ypj5hUJVSISS1jCvze74Y_9HcoAdhfAnbe_-N6B9C-nZ0</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>Chen, Jun-Song</creator><creator>Beckley, Janel R</creator><creator>McDonald, Nathan A</creator><creator>Ren, Liping</creator><creator>Mangione, MariaSanta</creator><creator>Jang, Sylvia J</creator><creator>Elmore, Zachary C</creator><creator>Rachfall, Nicole</creator><creator>Feoktistova, Anna</creator><creator>Jones, Christine M</creator><creator>Willet, Alaina H</creator><creator>Guillen, Rodrigo</creator><creator>Bitton, Danny A</creator><creator>Bähler, Jürg</creator><creator>Jensen, Michael A</creator><creator>Rhind, Nick</creator><creator>Gould, Kathleen L</creator><general>Oxford University Press</general><general>Genetics Society of America</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20150301</creationdate><title>Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains</title><author>Chen, Jun-Song ; Beckley, Janel R ; McDonald, Nathan A ; Ren, Liping ; Mangione, MariaSanta ; Jang, Sylvia J ; Elmore, Zachary C ; Rachfall, Nicole ; Feoktistova, Anna ; Jones, Christine M ; Willet, Alaina H ; Guillen, Rodrigo ; Bitton, Danny A ; Bähler, Jürg ; Jensen, Michael A ; Rhind, Nick ; Gould, Kathleen L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c556t-51f3ae198ae2870cda86b1058abf0431c49f3e04b53ee5552a3c7ba35da75cd73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Cell Division - genetics</topic><topic>DNA Damage - genetics</topic><topic>Gene Deletion</topic><topic>Genes, Fungal</topic><topic>Investigations</topic><topic>Schizosaccharomyces - cytology</topic><topic>Schizosaccharomyces - genetics</topic><topic>Schizosaccharomyces - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Jun-Song</creatorcontrib><creatorcontrib>Beckley, Janel R</creatorcontrib><creatorcontrib>McDonald, Nathan A</creatorcontrib><creatorcontrib>Ren, Liping</creatorcontrib><creatorcontrib>Mangione, MariaSanta</creatorcontrib><creatorcontrib>Jang, Sylvia J</creatorcontrib><creatorcontrib>Elmore, Zachary C</creatorcontrib><creatorcontrib>Rachfall, Nicole</creatorcontrib><creatorcontrib>Feoktistova, Anna</creatorcontrib><creatorcontrib>Jones, Christine M</creatorcontrib><creatorcontrib>Willet, Alaina H</creatorcontrib><creatorcontrib>Guillen, Rodrigo</creatorcontrib><creatorcontrib>Bitton, Danny A</creatorcontrib><creatorcontrib>Bähler, Jürg</creatorcontrib><creatorcontrib>Jensen, Michael A</creatorcontrib><creatorcontrib>Rhind, Nick</creatorcontrib><creatorcontrib>Gould, Kathleen L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>G3 : genes - genomes - genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Jun-Song</au><au>Beckley, Janel R</au><au>McDonald, Nathan A</au><au>Ren, Liping</au><au>Mangione, MariaSanta</au><au>Jang, Sylvia J</au><au>Elmore, Zachary C</au><au>Rachfall, Nicole</au><au>Feoktistova, Anna</au><au>Jones, Christine M</au><au>Willet, Alaina H</au><au>Guillen, Rodrigo</au><au>Bitton, Danny A</au><au>Bähler, Jürg</au><au>Jensen, Michael A</au><au>Rhind, Nick</au><au>Gould, Kathleen L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains</atitle><jtitle>G3 : genes - genomes - genetics</jtitle><addtitle>G3 (Bethesda)</addtitle><date>2015-03-01</date><risdate>2015</risdate><volume>5</volume><issue>3</issue><spage>361</spage><epage>370</epage><pages>361-370</pages><issn>2160-1836</issn><eissn>2160-1836</eissn><abstract>Abstract
Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of different stress conditions. We identified new genes involved in DNA metabolism, completion of the cell cycle, and morphogenesis. This subset of nonessential gene deletions will add to the toolkits available for the study of biological processes in S. pombe.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>25552606</pmid><doi>10.1534/g3.114.015701</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | G3 : genes - genomes - genetics, 2015-03, Vol.5 (3), p.361-370 |
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| language | eng |
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| source | Open Access: PubMed Central; Open Access: Oxford University Press Open Journals |
| subjects | Cell Division - genetics DNA Damage - genetics Gene Deletion Genes, Fungal Investigations Schizosaccharomyces - cytology Schizosaccharomyces - genetics Schizosaccharomyces - metabolism |
| title | Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains |
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