Comparative evaluation of the protective efficacy of two formulations of a recombinant Chlamydia abortus subunit candidate vaccine in a mouse model

Highlights • The ability of recombinant C. abortus Pmp18D formulated in CpG + FL adjuvants or Vibrio cholerae ghosts (VCGs) to induce innate and cross protective immunity against genital C. abortus infection was evaluated. • VCG-delivered rPmp18D was more effective than CpG + FL in stimulating the D...

Full description

Saved in:
Bibliographic Details
Published in:Vaccine 2015-04, Vol.33 (15), p.1865-1872
Main Authors: Pan, Qing, Pais, Roshan, Ohandjo, Adaugo, He, Cheng, He, Qing, Omosun, Yusuf, Igietseme, J.U, Eko, F.O
Format: Article
Language:English
Subjects:
Abortion
adjuvants
Adjuvants, Immunologic
Administration, Intranasal
Allergy and Immunology
Animal diseases
animal models
Animals
antibodies
Antibodies, Bacterial - immunology
Bacteria
Bacterial Vaccines - immunology
Chlamydia
Chlamydia - immunology
Chlamydia abortus
Chlamydia Infections - immunology
Chlamydia Infections - microbiology
Chlamydia Infections - prevention & control
Cloning
cross immunity
Cross Protection
Cytokines
Dendritic cells
Dendritic Cells - immunology
Disease Models, Animal
enzootic diseases
Enzyme-Linked Immunosorbent Assay
Female
Immunity
Immunity, Innate
Immunization
immunoglobulin A
inactivated vaccines
Infections
interferon-gamma
interleukin-1beta
interleukin-4
Kinases
Ligands
membrane proteins
Mice
Mice, Inbred C57BL
nose
Oligodeoxyribonucleotides - immunology
Pmp18D
pregnant women
Proteins
risk
secretion
Sexually transmitted diseases
sheep
STD
Studies
subunit vaccines
T-lymphocytes
T-Lymphocytes - immunology
Toll-Like Receptors - immunology
tumor necrosis factor-alpha
tyrosine
Vaccine delivery
Vaccines
Vaccines, Subunit - administration & dosage
Vaccines, Subunit - immunology
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - immunology
Vagina - microbiology
Vibrio cholerae
Vibrio cholerae - immunology
Vibrio cholerae - isolation & purification
Zoonoses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c616t-3c324f76ab30f6fa2f78fb387294d9268c019f1639bbb9ba69a9daebe9a0ed3d3
cites cdi_FETCH-LOGICAL-c616t-3c324f76ab30f6fa2f78fb387294d9268c019f1639bbb9ba69a9daebe9a0ed3d3
container_end_page 1872
container_issue 15
container_start_page 1865
container_title Vaccine
container_volume 33
creator Pan, Qing
Pais, Roshan
Ohandjo, Adaugo
He, Cheng
He, Qing
Omosun, Yusuf
Igietseme, J.U
Eko, F.O
description Highlights • The ability of recombinant C. abortus Pmp18D formulated in CpG + FL adjuvants or Vibrio cholerae ghosts (VCGs) to induce innate and cross protective immunity against genital C. abortus infection was evaluated. • VCG-delivered rPmp18D was more effective than CpG + FL in stimulating the DC expression of molecules critically involved in T cell activation and differentiation, and activation of innate immune signaling involving TLRs and NLRP3 inflammasome. • VCG-delivered rPmp18D elicited more robust antigen-specific cellular and humoral immune responses in mice compared to CpG + FL. • Vaccine delivery with VCG induced superior cross-protective immunity than delivery with a combination of CpG1826 and FL. • The vaccine delivery and immunomodulatory capacity of VCG is thus superior to that of CpG + FL adjuvants.
doi_str_mv 10.1016/j.vaccine.2015.02.007
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4380638</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0264410X15001735</els_id><sourcerecordid>3789460711</sourcerecordid><originalsourceid>FETCH-LOGICAL-c616t-3c324f76ab30f6fa2f78fb387294d9268c019f1639bbb9ba69a9daebe9a0ed3d3</originalsourceid><addsrcrecordid>eNqNks2O0zAUhSMEYsrAI4AssWHT4p_EiTeDRhV_0kgsAImddeNcU5fELnZS1OfghXGmZYDZDBvb8v3Ote7xKYqnjK4YZfLldrUHY5zHFaesWlG-orS-VyxYU4slr1hzv1hQLstlyeiXs-JRSltKaSWYelic8Uqqpmzkovi5DsMOIoxujwT30E_5GDwJlowbJLsYRjTHorXOgDlcl34EYkMcpv6aTvMdkIgmDK3z4Eey3vQwHDoHBNoQxymRNLWTdyMx4DvXwYjkNABxPouHMCXMa4f94-KBhT7hk9N-Xnx-8_rT-t3y6sPb9-vLq6WRTI5LYQQvbS2hFdRKC9zWjW1FU3NVdorLxlCmLJNCtW2rWpAKVAfYogKKnejEeXFx7Lub2gE7g36M0OtddAPEgw7g9L8V7zb6a9jrUjRUiiY3eHFqEMP3CdOoB5cM9j14zONonh1nolSU34kyKRteSsWq_0FrUVY1n9Hnt9BtmKLPpmlW07pquGQqU9WRMjGkFNHejMionsOkt_r0F3oOk6Zc5zBl3bO__blR_U5PBl4dAcy_tHcYdTIOvcHO5SyMugvuzicubnUwvfM5Z_03PGD6M41OWaA_zomeA82q7GwtKvELQBH2Iw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1707582619</pqid></control><display><type>article</type><title>Comparative evaluation of the protective efficacy of two formulations of a recombinant Chlamydia abortus subunit candidate vaccine in a mouse model</title><source>ScienceDirect Freedom Collection</source><creator>Pan, Qing ; Pais, Roshan ; Ohandjo, Adaugo ; He, Cheng ; He, Qing ; Omosun, Yusuf ; Igietseme, J.U ; Eko, F.O</creator><creatorcontrib>Pan, Qing ; Pais, Roshan ; Ohandjo, Adaugo ; He, Cheng ; He, Qing ; Omosun, Yusuf ; Igietseme, J.U ; Eko, F.O</creatorcontrib><description>Highlights • The ability of recombinant C. abortus Pmp18D formulated in CpG + FL adjuvants or Vibrio cholerae ghosts (VCGs) to induce innate and cross protective immunity against genital C. abortus infection was evaluated. • VCG-delivered rPmp18D was more effective than CpG + FL in stimulating the DC expression of molecules critically involved in T cell activation and differentiation, and activation of innate immune signaling involving TLRs and NLRP3 inflammasome. • VCG-delivered rPmp18D elicited more robust antigen-specific cellular and humoral immune responses in mice compared to CpG + FL. • Vaccine delivery with VCG induced superior cross-protective immunity than delivery with a combination of CpG1826 and FL. • The vaccine delivery and immunomodulatory capacity of VCG is thus superior to that of CpG + FL adjuvants.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2015.02.007</identifier><identifier>PMID: 25698486</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Abortion ; adjuvants ; Adjuvants, Immunologic ; Administration, Intranasal ; Allergy and Immunology ; Animal diseases ; animal models ; Animals ; antibodies ; Antibodies, Bacterial - immunology ; Bacteria ; Bacterial Vaccines - immunology ; Chlamydia ; Chlamydia - immunology ; Chlamydia abortus ; Chlamydia Infections - immunology ; Chlamydia Infections - microbiology ; Chlamydia Infections - prevention &amp; control ; Cloning ; cross immunity ; Cross Protection ; Cytokines ; Dendritic cells ; Dendritic Cells - immunology ; Disease Models, Animal ; enzootic diseases ; Enzyme-Linked Immunosorbent Assay ; Female ; Immunity ; Immunity, Innate ; Immunization ; immunoglobulin A ; inactivated vaccines ; Infections ; interferon-gamma ; interleukin-1beta ; interleukin-4 ; Kinases ; Ligands ; membrane proteins ; Mice ; Mice, Inbred C57BL ; nose ; Oligodeoxyribonucleotides - immunology ; Pmp18D ; pregnant women ; Proteins ; risk ; secretion ; Sexually transmitted diseases ; sheep ; STD ; Studies ; subunit vaccines ; T-lymphocytes ; T-Lymphocytes - immunology ; Toll-Like Receptors - immunology ; tumor necrosis factor-alpha ; tyrosine ; Vaccine delivery ; Vaccines ; Vaccines, Subunit - administration &amp; dosage ; Vaccines, Subunit - immunology ; Vaccines, Synthetic - administration &amp; dosage ; Vaccines, Synthetic - immunology ; Vagina - microbiology ; Vibrio cholerae ; Vibrio cholerae - immunology ; Vibrio cholerae - isolation &amp; purification ; Zoonoses</subject><ispartof>Vaccine, 2015-04, Vol.33 (15), p.1865-1872</ispartof><rights>Elsevier Ltd</rights><rights>2015 Elsevier Ltd</rights><rights>Copyright © 2015 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Apr 8, 2015</rights><rights>2015 Published by Elsevier Ltd. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c616t-3c324f76ab30f6fa2f78fb387294d9268c019f1639bbb9ba69a9daebe9a0ed3d3</citedby><cites>FETCH-LOGICAL-c616t-3c324f76ab30f6fa2f78fb387294d9268c019f1639bbb9ba69a9daebe9a0ed3d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25698486$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pan, Qing</creatorcontrib><creatorcontrib>Pais, Roshan</creatorcontrib><creatorcontrib>Ohandjo, Adaugo</creatorcontrib><creatorcontrib>He, Cheng</creatorcontrib><creatorcontrib>He, Qing</creatorcontrib><creatorcontrib>Omosun, Yusuf</creatorcontrib><creatorcontrib>Igietseme, J.U</creatorcontrib><creatorcontrib>Eko, F.O</creatorcontrib><title>Comparative evaluation of the protective efficacy of two formulations of a recombinant Chlamydia abortus subunit candidate vaccine in a mouse model</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Highlights • The ability of recombinant C. abortus Pmp18D formulated in CpG + FL adjuvants or Vibrio cholerae ghosts (VCGs) to induce innate and cross protective immunity against genital C. abortus infection was evaluated. • VCG-delivered rPmp18D was more effective than CpG + FL in stimulating the DC expression of molecules critically involved in T cell activation and differentiation, and activation of innate immune signaling involving TLRs and NLRP3 inflammasome. • VCG-delivered rPmp18D elicited more robust antigen-specific cellular and humoral immune responses in mice compared to CpG + FL. • Vaccine delivery with VCG induced superior cross-protective immunity than delivery with a combination of CpG1826 and FL. • The vaccine delivery and immunomodulatory capacity of VCG is thus superior to that of CpG + FL adjuvants.</description><subject>Abortion</subject><subject>adjuvants</subject><subject>Adjuvants, Immunologic</subject><subject>Administration, Intranasal</subject><subject>Allergy and Immunology</subject><subject>Animal diseases</subject><subject>animal models</subject><subject>Animals</subject><subject>antibodies</subject><subject>Antibodies, Bacterial - immunology</subject><subject>Bacteria</subject><subject>Bacterial Vaccines - immunology</subject><subject>Chlamydia</subject><subject>Chlamydia - immunology</subject><subject>Chlamydia abortus</subject><subject>Chlamydia Infections - immunology</subject><subject>Chlamydia Infections - microbiology</subject><subject>Chlamydia Infections - prevention &amp; control</subject><subject>Cloning</subject><subject>cross immunity</subject><subject>Cross Protection</subject><subject>Cytokines</subject><subject>Dendritic cells</subject><subject>Dendritic Cells - immunology</subject><subject>Disease Models, Animal</subject><subject>enzootic diseases</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Immunity</subject><subject>Immunity, Innate</subject><subject>Immunization</subject><subject>immunoglobulin A</subject><subject>inactivated vaccines</subject><subject>Infections</subject><subject>interferon-gamma</subject><subject>interleukin-1beta</subject><subject>interleukin-4</subject><subject>Kinases</subject><subject>Ligands</subject><subject>membrane proteins</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>nose</subject><subject>Oligodeoxyribonucleotides - immunology</subject><subject>Pmp18D</subject><subject>pregnant women</subject><subject>Proteins</subject><subject>risk</subject><subject>secretion</subject><subject>Sexually transmitted diseases</subject><subject>sheep</subject><subject>STD</subject><subject>Studies</subject><subject>subunit vaccines</subject><subject>T-lymphocytes</subject><subject>T-Lymphocytes - immunology</subject><subject>Toll-Like Receptors - immunology</subject><subject>tumor necrosis factor-alpha</subject><subject>tyrosine</subject><subject>Vaccine delivery</subject><subject>Vaccines</subject><subject>Vaccines, Subunit - administration &amp; dosage</subject><subject>Vaccines, Subunit - immunology</subject><subject>Vaccines, Synthetic - administration &amp; dosage</subject><subject>Vaccines, Synthetic - immunology</subject><subject>Vagina - microbiology</subject><subject>Vibrio cholerae</subject><subject>Vibrio cholerae - immunology</subject><subject>Vibrio cholerae - isolation &amp; purification</subject><subject>Zoonoses</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNks2O0zAUhSMEYsrAI4AssWHT4p_EiTeDRhV_0kgsAImddeNcU5fELnZS1OfghXGmZYDZDBvb8v3Ote7xKYqnjK4YZfLldrUHY5zHFaesWlG-orS-VyxYU4slr1hzv1hQLstlyeiXs-JRSltKaSWYelic8Uqqpmzkovi5DsMOIoxujwT30E_5GDwJlowbJLsYRjTHorXOgDlcl34EYkMcpv6aTvMdkIgmDK3z4Eey3vQwHDoHBNoQxymRNLWTdyMx4DvXwYjkNABxPouHMCXMa4f94-KBhT7hk9N-Xnx-8_rT-t3y6sPb9-vLq6WRTI5LYQQvbS2hFdRKC9zWjW1FU3NVdorLxlCmLJNCtW2rWpAKVAfYogKKnejEeXFx7Lub2gE7g36M0OtddAPEgw7g9L8V7zb6a9jrUjRUiiY3eHFqEMP3CdOoB5cM9j14zONonh1nolSU34kyKRteSsWq_0FrUVY1n9Hnt9BtmKLPpmlW07pquGQqU9WRMjGkFNHejMionsOkt_r0F3oOk6Zc5zBl3bO__blR_U5PBl4dAcy_tHcYdTIOvcHO5SyMugvuzicubnUwvfM5Z_03PGD6M41OWaA_zomeA82q7GwtKvELQBH2Iw</recordid><startdate>20150408</startdate><enddate>20150408</enddate><creator>Pan, Qing</creator><creator>Pais, Roshan</creator><creator>Ohandjo, Adaugo</creator><creator>He, Cheng</creator><creator>He, Qing</creator><creator>Omosun, Yusuf</creator><creator>Igietseme, J.U</creator><creator>Eko, F.O</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20150408</creationdate><title>Comparative evaluation of the protective efficacy of two formulations of a recombinant Chlamydia abortus subunit candidate vaccine in a mouse model</title><author>Pan, Qing ; Pais, Roshan ; Ohandjo, Adaugo ; He, Cheng ; He, Qing ; Omosun, Yusuf ; Igietseme, J.U ; Eko, F.O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c616t-3c324f76ab30f6fa2f78fb387294d9268c019f1639bbb9ba69a9daebe9a0ed3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Abortion</topic><topic>adjuvants</topic><topic>Adjuvants, Immunologic</topic><topic>Administration, Intranasal</topic><topic>Allergy and Immunology</topic><topic>Animal diseases</topic><topic>animal models</topic><topic>Animals</topic><topic>antibodies</topic><topic>Antibodies, Bacterial - immunology</topic><topic>Bacteria</topic><topic>Bacterial Vaccines - immunology</topic><topic>Chlamydia</topic><topic>Chlamydia - immunology</topic><topic>Chlamydia abortus</topic><topic>Chlamydia Infections - immunology</topic><topic>Chlamydia Infections - microbiology</topic><topic>Chlamydia Infections - prevention &amp; control</topic><topic>Cloning</topic><topic>cross immunity</topic><topic>Cross Protection</topic><topic>Cytokines</topic><topic>Dendritic cells</topic><topic>Dendritic Cells - immunology</topic><topic>Disease Models, Animal</topic><topic>enzootic diseases</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Immunity</topic><topic>Immunity, Innate</topic><topic>Immunization</topic><topic>immunoglobulin A</topic><topic>inactivated vaccines</topic><topic>Infections</topic><topic>interferon-gamma</topic><topic>interleukin-1beta</topic><topic>interleukin-4</topic><topic>Kinases</topic><topic>Ligands</topic><topic>membrane proteins</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>nose</topic><topic>Oligodeoxyribonucleotides - immunology</topic><topic>Pmp18D</topic><topic>pregnant women</topic><topic>Proteins</topic><topic>risk</topic><topic>secretion</topic><topic>Sexually transmitted diseases</topic><topic>sheep</topic><topic>STD</topic><topic>Studies</topic><topic>subunit vaccines</topic><topic>T-lymphocytes</topic><topic>T-Lymphocytes - immunology</topic><topic>Toll-Like Receptors - immunology</topic><topic>tumor necrosis factor-alpha</topic><topic>tyrosine</topic><topic>Vaccine delivery</topic><topic>Vaccines</topic><topic>Vaccines, Subunit - administration &amp; dosage</topic><topic>Vaccines, Subunit - immunology</topic><topic>Vaccines, Synthetic - administration &amp; dosage</topic><topic>Vaccines, Synthetic - immunology</topic><topic>Vagina - microbiology</topic><topic>Vibrio cholerae</topic><topic>Vibrio cholerae - immunology</topic><topic>Vibrio cholerae - isolation &amp; purification</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pan, Qing</creatorcontrib><creatorcontrib>Pais, Roshan</creatorcontrib><creatorcontrib>Ohandjo, Adaugo</creatorcontrib><creatorcontrib>He, Cheng</creatorcontrib><creatorcontrib>He, Qing</creatorcontrib><creatorcontrib>Omosun, Yusuf</creatorcontrib><creatorcontrib>Igietseme, J.U</creatorcontrib><creatorcontrib>Eko, F.O</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pan, Qing</au><au>Pais, Roshan</au><au>Ohandjo, Adaugo</au><au>He, Cheng</au><au>He, Qing</au><au>Omosun, Yusuf</au><au>Igietseme, J.U</au><au>Eko, F.O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative evaluation of the protective efficacy of two formulations of a recombinant Chlamydia abortus subunit candidate vaccine in a mouse model</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2015-04-08</date><risdate>2015</risdate><volume>33</volume><issue>15</issue><spage>1865</spage><epage>1872</epage><pages>1865-1872</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>Highlights • The ability of recombinant C. abortus Pmp18D formulated in CpG + FL adjuvants or Vibrio cholerae ghosts (VCGs) to induce innate and cross protective immunity against genital C. abortus infection was evaluated. • VCG-delivered rPmp18D was more effective than CpG + FL in stimulating the DC expression of molecules critically involved in T cell activation and differentiation, and activation of innate immune signaling involving TLRs and NLRP3 inflammasome. • VCG-delivered rPmp18D elicited more robust antigen-specific cellular and humoral immune responses in mice compared to CpG + FL. • Vaccine delivery with VCG induced superior cross-protective immunity than delivery with a combination of CpG1826 and FL. • The vaccine delivery and immunomodulatory capacity of VCG is thus superior to that of CpG + FL adjuvants.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>25698486</pmid><doi>10.1016/j.vaccine.2015.02.007</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0264-410X
ispartof Vaccine, 2015-04, Vol.33 (15), p.1865-1872
issn 0264-410X
1873-2518
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4380638
source ScienceDirect Freedom Collection
subjects Abortion
adjuvants
Adjuvants, Immunologic
Administration, Intranasal
Allergy and Immunology
Animal diseases
animal models
Animals
antibodies
Antibodies, Bacterial - immunology
Bacteria
Bacterial Vaccines - immunology
Chlamydia
Chlamydia - immunology
Chlamydia abortus
Chlamydia Infections - immunology
Chlamydia Infections - microbiology
Chlamydia Infections - prevention & control
Cloning
cross immunity
Cross Protection
Cytokines
Dendritic cells
Dendritic Cells - immunology
Disease Models, Animal
enzootic diseases
Enzyme-Linked Immunosorbent Assay
Female
Immunity
Immunity, Innate
Immunization
immunoglobulin A
inactivated vaccines
Infections
interferon-gamma
interleukin-1beta
interleukin-4
Kinases
Ligands
membrane proteins
Mice
Mice, Inbred C57BL
nose
Oligodeoxyribonucleotides - immunology
Pmp18D
pregnant women
Proteins
risk
secretion
Sexually transmitted diseases
sheep
STD
Studies
subunit vaccines
T-lymphocytes
T-Lymphocytes - immunology
Toll-Like Receptors - immunology
tumor necrosis factor-alpha
tyrosine
Vaccine delivery
Vaccines
Vaccines, Subunit - administration & dosage
Vaccines, Subunit - immunology
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - immunology
Vagina - microbiology
Vibrio cholerae
Vibrio cholerae - immunology
Vibrio cholerae - isolation & purification
Zoonoses
title Comparative evaluation of the protective efficacy of two formulations of a recombinant Chlamydia abortus subunit candidate vaccine in a mouse model
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T22%3A23%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20evaluation%20of%20the%20protective%20efficacy%20of%20two%20formulations%20of%20a%20recombinant%20Chlamydia%20abortus%20subunit%20candidate%20vaccine%20in%20a%20mouse%20model&rft.jtitle=Vaccine&rft.au=Pan,%20Qing&rft.date=2015-04-08&rft.volume=33&rft.issue=15&rft.spage=1865&rft.epage=1872&rft.pages=1865-1872&rft.issn=0264-410X&rft.eissn=1873-2518&rft_id=info:doi/10.1016/j.vaccine.2015.02.007&rft_dat=%3Cproquest_pubme%3E3789460711%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c616t-3c324f76ab30f6fa2f78fb387294d9268c019f1639bbb9ba69a9daebe9a0ed3d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1707582619&rft_id=info:pmid/25698486&rfr_iscdi=true