CYR61 (CCN1) overexpression induces lung injury in mice

Cysteine-rich protein-61 (CYR61), also known as connective tissue growth factor, CYR61, and nephroblastoma overexpressed gene 1 (CCN1), is a heparin-binding protein member of the CCN family of matricellular proteins. Gene expression profiles showed that Cyr61 is upregulated in human acute lung injur...

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Published in:American journal of physiology. Lung cellular and molecular physiology 2015-04, Vol.308 (8), p.L759-L765
Main Authors: Grazioli, Serge, Gil, Sucheol, An, Dowon, Kajikawa, Osamu, Farnand, Alex W, Hanson, Josiah F, Birkland, Timothy, Chen, Peter, Duffield, Jeremy, Schnapp, Lynn M, Altemeier, William A, Matute-Bello, Gustavo
Format: Article
Language:English
Subjects:
Acute Lung Injury - metabolism
Adenovirus
Adenoviruses
Animals
Binding sites
Call for Papers
Cysteine-Rich Protein 61 - genetics
Cysteine-Rich Protein 61 - metabolism
Gene Expression
Humans
Lung - metabolism
Lung diseases
Male
Mice, Inbred C57BL
Physiology
Pulmonary fibrosis
Respiratory Distress Syndrome - metabolism
Rodents
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Summary:Cysteine-rich protein-61 (CYR61), also known as connective tissue growth factor, CYR61, and nephroblastoma overexpressed gene 1 (CCN1), is a heparin-binding protein member of the CCN family of matricellular proteins. Gene expression profiles showed that Cyr61 is upregulated in human acute lung injury (ALI), but its functional role is unclear. We hypothesized that CYR61 contributes to ALI in mice. First, we demonstrated that CYR61 expression increases after bleomycin-induced lung injury. We then used adenovirus-mediated gene transfer to determine whether CYR61 overexpression in the lungs was sufficient to cause ALI. Mice instilled with CYR61 adenovirus showed greater weight loss, increased bronchoalveolar lavage total neutrophil counts, increased protein concentrations, and increased mortality compared with mice instilled with empty-vector adenovirus. Immunohistochemical studies in lungs from humans with idiopathic pulmonary fibrosis revealed CYR61 expression on the luminal membrane of alveolar epithelial cells in areas of injury. We conclude that CYR61 is upregulated in ALI and that CYR61 overexpression exacerbates ALI in mice.
ISSN:1040-0605
1522-1504
DOI:10.1152/ajplung.00190.2014