Lipopolysaccharide composition of the wilt pathogen, Pseudomonas solanacearum: Correlation with the hypersensitive response in tobacco

In the induction of the hypersensitive response (HR) in tobacco by Pseudomonas solanacearum, the recognition between host and pathogen is thought to involve an interaction between plant lectins and bacterial lipopolysaccharide (LPS). The LPS of a series of strains of P. solanacearum were examined to...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1980-03, Vol.65 (3), p.557-559
Main Authors: Whatley, Mariamne H., Hunter, Nora, Cantrell, Michael A., Hendrick, Carol, Keegstra, Kenneth, Sequeira, Luis
Format: Article
Language:English
Subjects:
Bacteria
Bacteriophages
Cell walls
Electrophoresis
Gels
Hypersensitive response
Lectins
Lipopolysaccharides
Ralstonia solanacearum
Short Communication
Short Communications
Sugars
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Summary:In the induction of the hypersensitive response (HR) in tobacco by Pseudomonas solanacearum, the recognition between host and pathogen is thought to involve an interaction between plant lectins and bacterial lipopolysaccharide (LPS). The LPS of a series of strains of P. solanacearum were examined to determine if there are structural differences that might account for the ability or inability of these strains to induce the hypersensitive response. Analysis of the components of LPS by gas chromatography indicates a clear difference in sugar composition between the HR-inducing and non-HR-inducing strains, especially in terms of the percentage of glucose, xylose and rhamnose. Sodium dodecyl sulfate polyacrylamide gel electrophoresis shows there are two distinct kinds of LPS, differing greatly in size, which correspond to rough and smooth LPS in other systems. In addition, a phage, CH154, was isolated which lyses non-HR-inducing bacteria and which is inactivated by LPS from these bacterial strains. Therefore, differences in LPS structure correlate strongly with host recognition of Pseudomonas solanacearum.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.65.3.557