Cisplatin combined with hyperthermia kills HepG2 cells in intraoperative blood salvage but preserves the function of erythrocytes

The safe use of intraoperative blood salvage (IBS) in cancer surgery remains controversial. Here, we investigated the killing effect of cisplatin combined with hyperthermia on human hepatocarcinoma (HepG2) cells and erythrocytes from IBS in vitro . HepG2 cells were mixed with concentrated erythrocyt...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2015-05, Vol.16 (5), p.395-403
Main Authors: Yang, Jin-ting, Tang, Li-hui, Liu, Yun-qing, Wang, Yin, Wang, Lie-ju, Zhang, Feng-jiang, Yan, Min
Format: Article
Language:English
Subjects:
2,3-Diphosphoglycerate - chemistry
Adult
Aged
Antineoplastic Agents - therapeutic use
Biomedical and Life Sciences
Biomedicine
Cell Survival
Cisplatin - therapeutic use
Combined Modality Therapy
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Erythrocytes - drug effects
Female
Hemoglobins - chemistry
Hep G2 Cells
Humans
Hyperthermia, Induced
Male
Middle Aged
Operative Blood Salvage
Osmosis
Phosphatidylserines - chemistry
Phospholipids - chemistry
Sodium-Potassium-Exchanging ATPase - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The safe use of intraoperative blood salvage (IBS) in cancer surgery remains controversial. Here, we investigated the killing effect of cisplatin combined with hyperthermia on human hepatocarcinoma (HepG2) cells and erythrocytes from IBS in vitro . HepG2 cells were mixed with concentrated erythrocytes and pretreated with cisplatin (50, 100, and 200 μg/ml) alone at 37 °C for 60 min and cisplatin (25, 50, 100, and 200 μg/ml) combined with hyperthermia at 42 °C for 60 min. After pretreatment, the cell viability, colony formation and DNA metabolism in HepG2 and the Na + -K + -ATPase activity, 2,3-diphosphoglycerate (2,3-DPG) concentration, free hemoglobin (Hb) level, osmotic fragility, membrane phosphatidylserine externalization, and blood gas variables in erythrocytes were determined. Pretreatment with cisplatin (50, 100, and 200 μg/ml) combined with hyperthermia (42 °C) for 60 min significantly decreased HepG2 cell viability, and completely inhibited colony formation and DNA metabolism when the HepG2 cell concentration was 5×10 4 ml −1 in the erythrocyte ( P
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B1400224