Real-time quantitative monitoring of hiPSC-based model of macular degeneration on Electric Cell-substrate Impedance Sensing microelectrodes

Age-related macular degeneration (AMD) is the leading cause of blindness in the developed world. Humanized disease models are required to develop new therapies for currently incurable forms of AMD. In this work, a tissue-on-a-chip approach was developed through combining human induced pluripotent st...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2015-09, Vol.71, p.445-455
Main Authors: Gamal, W., Borooah, S., Smith, S., Underwood, I., Srsen, V., Chandran, S., Bagnaninchi, P.O., Dhillon, B.
Format: Article
Language:English
Subjects:
Adhesion
Assaying
Biological Assay - instrumentation
Biotechnology
Cell Movement
Cells, Cultured
Computer Systems
Damage
Degeneration
Dielectric Spectroscopy - instrumentation
Disease model
Equipment Design
Equipment Failure Analysis
Human induced pluripotent stem cells
Humans
Impedance sensing
Macular degeneration
Macular Degeneration - pathology
Macular Degeneration - physiopathology
Microelectrodes
Platforms
Real time
Reproducibility of Results
Retinal Pigment Epithelium - pathology
Retinal Pigment Epithelium - physiology
Sensitivity and Specificity
Therapy
Tissue Array Analysis - instrumentation
Tissue-on-a-chip
Wound healing
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Summary:Age-related macular degeneration (AMD) is the leading cause of blindness in the developed world. Humanized disease models are required to develop new therapies for currently incurable forms of AMD. In this work, a tissue-on-a-chip approach was developed through combining human induced pluripotent stem cells, Electric Cell–substrate Impedance Sensing (ECIS) and reproducible electrical wounding assays to model and quantitatively study AMD. Retinal Pigment Epithelium (RPE) cells generated from a patient with an inherited macular degeneration and from an unaffected sibling were used to test the model platform on which a reproducible electrical wounding assay was conducted to model RPE damage. First, a robust and reproducible real-time quantitative monitoring over a 25-day period demonstrated the establishment and maturation of RPE layers on the microelectrode arrays. A spatially controlled RPE layer damage that mimicked cell loss in AMD disease was then initiated. Post recovery, significant differences (P
ISSN:0956-5663
1873-4235
1873-4235
DOI:10.1016/j.bios.2015.04.079