Healing activity of Casearia sylvestris Sw. in second-degree scald burns in rodents

• Published in: BMC research notes 2015-06, Vol.8 (1), p.269, Article 269
• Main Authors: de Campos, Evandro Pedro, Trombini, Letícia Nava, Rodrigues, Rafaela, Portella, Décio Luis, Werner, Adriana Carolina, Ferraz, Miriele Cristina, de Oliveira, Robson Vicente Machado, Cogo, José Carlos, Oshima-Franco, Yoko, Aranha, Norberto, Gerenutti, Marli
• Format: Article
• Language: English
• Subjects: Analgesics - isolation & purification; Analgesics - pharmacology; Animals; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; Antivenins - isolation & purification; Antivenins - pharmacology; Bothrops - metabolism; Burns - drug therapy; Burns - pathology; Casearia - chemistry; Chromatography; Crotalid Venoms - antagonists & inhibitors; Crotalid Venoms - isolation & purification; Crotalid Venoms - toxicity; Health aspects; Hot Temperature; Male; Mice; Neovascularization; Neuroprotective Agents - isolation & purification; Neuroprotective Agents - pharmacology; Plant Extracts - chemistry; Rats, Wistar; Skin - drug effects; Skin - injuries; Skin - pathology; Snakes; Venom; Wound Healing - drug effects
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-015-1251-4

Every year thousands of people are victims of burns, mainly scald burns. Many of these victims have small size wounds and superficial partial thickness and do not seek specialized medical care. As in Brazil Casearia sylvestris Sw., popularly known as guaçatonga is widely used for its analgesic, antiseptic and anti-inflammatory activities, this study sought to evaluate the effects of its hydroalcoholic extract in healing process of burns injuries. The obtained extract was validated applying a thin layer chromatography and sophisticated validation method using Bothrops jararacussu snake venom that is necrotic and inflammatory, and by which guaçatonga extract was able to neutralize the irreversible neuromuscular blockade induced by the venom. After induction of the scald injury, the animals were treated daily with saline solution spray; spray containing extract; biofilm; or biofilm impregnated with extract. Significant differences were observed between the four groups studied considering: extension of the healing area, neovascularization, fibroblast proliferation, and epithelialization. The anti-inflammatory and bactericidal effects of C. sylvestris Sw. suggests a potential therapeutic benefit in the treatment of inflammatory conditions in second-degree scald burn injuries, as well as, counteracting against the in vitro paralysis induced by B. jararacussu venom.