Der1, a novel protein specifically required for endoplasmic reticulum degradation in yeast

The endoplasmic reticulum (ER) of the yeast Saccharomyces cerevisiae contains a proteolytic system able to selectively degrade misfolded lumenal secretory proteins. For examination of the components involved in this degradation process, mutants were isolated. They could be divided into four compleme...

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Bibliographic Details
Published in:The EMBO journal 1996-02, Vol.15 (4), p.753-763
Main Authors: Knop, M, Finger, A, Braun, T, Hellmuth, K, Wolf, D.H
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Cloning, Molecular
degradation
der1 gene
endoplasmic reticulum
Endoplasmic Reticulum - metabolism
Fungal Proteins - metabolism
genbank/x92435
Gene Expression
Genes, Fungal
Membrane Proteins - genetics
Membrane Proteins - metabolism
messenger RNA
Molecular Sequence Data
mutants
nucleotide sequences
protein degradation
proteins
RNA, Messenger - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Sequence Alignment
Sequence Homology, Amino Acid
structural genes
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Summary:The endoplasmic reticulum (ER) of the yeast Saccharomyces cerevisiae contains a proteolytic system able to selectively degrade misfolded lumenal secretory proteins. For examination of the components involved in this degradation process, mutants were isolated. They could be divided into four complementation groups. The mutations led to stabilization of two different substrates for this process. The mutant classes were called 'der' for 'degradation in the ER'. DER1 was cloned by complementation of the der1-2 mutation. The DER1 gene codes for a novel, hydrophobic protein, that is localized to the ER. Deletion of DER1 abolished degradation of the substrate proteins. The function of the Der1 protein seems to be specifically required for the degradation process associated with the ER. The depletion of Der1 from cells causes neither detectable growth phenotypes nor a general accumulation of unfolded proteins in the ER. In DER1 deleted cells, a substrate protein for ER degradation is retained in the ER by the same mechanism which also retains lumenal ER residents. This suggests that DER1 acts in a process that directly removes protein from the folding environment of the ER.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1996.tb00411.x