Intrinsically disordered proteins drive membrane curvature

• Published in: Nature communications 2015-07, Vol.6 (1), p.7875-7875, Article 7875
• Main Authors: Busch, David J., Houser, Justin R., Hayden, Carl C., Sherman, Michael B., Lafer, Eileen M., Stachowiak, Jeanne C.
• Format: Article
• Language: English
• Subjects: 14/19; 14/28; 631/57/2271; 631/80/313/2375; 82/80; Adaptor Proteins, Vesicular Transport - metabolism; Cell Membrane - physiology; Cell Shape; Cells, Cultured; Clathrin-Coated Vesicles - physiology; Humanities and Social Sciences; Humans; Intrinsically Disordered Proteins - physiology; Monomeric Clathrin Assembly Proteins - metabolism; multidisciplinary; Protein Structure, Tertiary; Science; Science (multidisciplinary)
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms8875

Assembly of highly curved membrane structures is essential to cellular physiology. The prevailing view has been that proteins with curvature-promoting structural motifs, such as wedge-like amphipathic helices and crescent-shaped BAR domains, are required for bending membranes. Here we report that intrinsically disordered domains of the endocytic adaptor proteins, Epsin1 and AP180 are highly potent drivers of membrane curvature. This result is unexpected since intrinsically disordered domains lack a well-defined three-dimensional structure. However, in vitro measurements of membrane curvature and protein diffusivity demonstrate that the large hydrodynamic radii of these domains generate steric pressure that drives membrane bending. When disordered adaptor domains are expressed as transmembrane cargo in mammalian cells, they are excluded from clathrin-coated pits. We propose that a balance of steric pressure on the two surfaces of the membrane drives this exclusion. These results provide quantitative evidence for the influence of steric pressure on the content and assembly of curved cellular membrane structures. Proteins that bend membranes often contain curvature-promoting structural motifs such as wedges or crescent-shaped domains. Busch et al. report that intrinsically disordered domains can also drive membrane curvature and provide evidence that steric pressure driven by protein crowding mediates this effect.