A novel oncogene related to c‐mil is transduced in chicken neuroretina cells induced to proliferate by infection with an avian lymphomatosis virus

Non‐dividing neuroretina cells from chicken embryos are induced to proliferate after a long latency, following infection with Rous associated virus type 1, an avian retrovirus which does not carry a transforming gene. We have isolated from these proliferating cells an acutely mitogenic retrovirus, d...

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Bibliographic Details
Published in:The EMBO journal 1988-11, Vol.7 (11), p.3369-3373
Main Authors: Marx, M., Eychène, A., Laugier, D., Béchade, C., Crisanti, P., Dezélée, P., Pessac, B., Calothy, G.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Avian Leukosis Virus - physiology
Base Sequence
Biological and medical sciences
Blotting, Southern
Cell Transformation, Viral
Cells, Cultured
Chick Embryo
Cloning, Molecular
Deoxyribonuclease EcoRI
DNA - genetics
Electrophoresis, Agar Gel
Fundamental and applied biological sciences. Psychology
Microbiology
Molecular Sequence Data
Oncogene Proteins v-raf
Oncogenes
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Restriction Mapping
Retroviridae Proteins - genetics
Transduction, Genetic
Virology
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Summary:Non‐dividing neuroretina cells from chicken embryos are induced to proliferate after a long latency, following infection with Rous associated virus type 1, an avian retrovirus which does not carry a transforming gene. We have isolated from these proliferating cells an acutely mitogenic retrovirus, designated IC10, which contains a novel oncogene. Nucleotide sequencing showed that the IC10 virus has transduced 1101 nucleotides of cellular origin inserted between the gag and env genes of RAV‐1. This oncogene, designated v‐Rmil, is 70.1% homologous to v‐mil. v‐Rmil encodes a protein of 40,976 daltons sharing 83.8% homology with the catalytic domain of the v‐mil protein. Divergence with the v‐mil gene product is observed at the NH2‐ and COOH‐terminal portions of the v‐Rmil protein. Restriction analysis of normal chicken DNA indicated that v‐Rmil is derived from a cellular gene distinct from c‐mil. The c‐Rmil gene is transcribed through a major mRNA, greater than 10 kb in length, that is detected at much higher levels in neuroretinas, as compared to other embryonic tissues.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1988.tb03209.x