Effect of retinoic acid on cell proliferation kinetics and retinoic acid receptor expression of colorectal mucosa

• Published in: World journal of gastroenterology : WJG 2003-08, Vol.9 (8), p.1725-1728
• Main Authors: Wei, Hong-Bo, Han, Xiao-Yan, Fan, Wei, Chen, Gui-Hua, Wang, Ji-Fu
• Format: Article
• Language: English
• Subjects: Animals; Antineoplastic Agents - pharmacology; Cell Division - drug effects; Colon - cytology; Colon - metabolism; Colorectal Cancer; Intestinal Mucosa - cytology; Intestinal Mucosa - metabolism; Kinetics; Male; Rats; Rats, Wistar; Receptors, Retinoic Acid - metabolism; Rectum - cytology; Rectum - metabolism; Tretinoin - pharmacology; 增殖细胞核抗原; 细胞增殖; 结肠直肠癌; 维甲酸
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v9.i8.1725

AIM: To investigate the effect of retinoic acid (RA) on cell proliferation kinetics and retinoic acid receptor (PAR) expression of colorectal mucosa.METHODS: One hundred sixty healthy male Wistar rats were randomly divided into 4 groups. Rats in groups I and Ⅱ were subcutaneously injected with dimethylhydrazine (DNH) (20 mg/kg, once a week,) for 7 to 13 weeks, while groups Ⅲ and IV were injected with normal saline. Rats in groups Ⅱ and Ⅲ were also treated with RA (50 mg/kg,every day, orally) from 7th to 15th week, thus group IV was used as a control. The rats were killed in different batches.The expressions of proliferating cell nuclear antigen (PCNA),nucleolar organizer region-associated protein (AgNOR) and RAR were detected.RESULTS: The incidence of colorectal carcinoma was different between groups I (100 %) and Ⅱ (15 %) (P