Tetraspanins CD9 and CD151 at the immune synapse support T‐cell integrin signaling

Understanding how the immune response is activated and amplified requires detailed knowledge of the stages in the formation of the immunological synapse (IS) between T lymphocytes and antigen‐presenting cells (APCs). We show that tetraspanins CD9 and CD151 congregate at the T‐cell side of the IS. Si...

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Bibliographic Details
Published in:European journal of immunology 2014-07, Vol.44 (7), p.1967-1975
Main Authors: Rocha‐Perugini, Vera, González‐Granado, José Maria, Tejera, Emilio, López‐Martín, Soraya, Yañez‐Mó, Maria, Sánchez‐Madrid, Francisco
Format: Article
Language:English
Subjects:
CD151
CD9
Humans
Immune synapse
Immunological Synapses - immunology
Integrins
Integrins - physiology
Jurkat Cells
Lymphocyte Activation
Signal Transduction - physiology
T cell receptors
T-Lymphocytes - immunology
Tetraspanin 24 - physiology
Tetraspanin 29 - physiology
T‐cell activation
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Summary:Understanding how the immune response is activated and amplified requires detailed knowledge of the stages in the formation of the immunological synapse (IS) between T lymphocytes and antigen‐presenting cells (APCs). We show that tetraspanins CD9 and CD151 congregate at the T‐cell side of the IS. Silencing of CD9 or CD151 blunts the IL‐2 secretion and expression of the activation marker CD69 by APC‐conjugated T lymphocytes, but does not affect the accumulation of CD3 or actin to the IS, or the translocation of the microtubule‐organizing center toward the T‐B contact area. CD9 or CD151 silencing diminishes the relocalization of α4β1 integrin to the IS and reduces the accumulation of high‐affinity β1 integrins at the cell–cell contact. These changes are accompanied by diminished phosphorylation of the integrin downstream targets FAK and ERK1/2. Our results suggest that CD9 and CD151 support integrin‐mediated signaling at the IS.
ISSN:0014-2980
1521-4141
DOI:10.1002/eji.201344235