Relationship between plasma D(-)-lactate and intestinal damage after severe injuries in rats

To explore the kinetic changes in plasma D(-)-lactate and lipopolysaccharide (LPS) levels, and investigate whether D(-)-lactate could be used as a marker of intestinal injury in rats following gut ischemia/reperfusion, burn, and acute necrotizing pancreatitis (ANP). Three models were developed in ra...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2001-08, Vol.7 (4), p.555-558
Main Authors: Sun, X Q, Fu, X B, Zhang, R, Lu, Y, Deng, Q, Jiang, X G, Sheng, Z Y
Format: Article
Language:English
Subjects:
Animals
Biomarkers
Burns - blood
Disease Models, Animal
Intestines - pathology
Ischemia - blood
Ischemia - pathology
Kinetics
Lactic Acid - blood
Lipopolysaccharides - blood
Male
Original Research
Pancreatitis, Acute Necrotizing - blood
Rats
Rats, Wistar
Reperfusion
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Summary:To explore the kinetic changes in plasma D(-)-lactate and lipopolysaccharide (LPS) levels, and investigate whether D(-)-lactate could be used as a marker of intestinal injury in rats following gut ischemia/reperfusion, burn, and acute necrotizing pancreatitis (ANP). Three models were developed in rats: (1)gut ischemia/reperfusion obtained by one hour of superior mesenteric artery occlusion followed by reperfusion; (2)severe burn injury created by 30% of total body surface area (TBSA) full-thickness scald burn; and (3)ANP induced by continuous inverse infusion of sodium taurocholate and trypsin into main pancreatic duct. Plasma levels of D(-)-lactate in systemic circulation and LPS in portal circulation were measured by enzymatic-spectrophotometric method and limulus amebocyte lysate (LAL) test kit, respectively. Tissue samples of intestine were taken for histological analysis. One hour gut ischemia followed by reperfusion injuries resulted in a significant elevation in plasma D(-)-lactate and LPS levels, and there was a significant correlation between the plasma D(-)-lactate and LPS (r = 0.719, P
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v7.i4.555