Influence of interleukin-1 beta gene polymorphisms on the risk of myocardial infarction and ischemic stroke at young age in vivo and in vitro

• Published in: International journal of clinical and experimental pathology 2015-01, Vol.8 (11), p.13806-13813
• Main Authors: Yang, Bo, Zhao, Hua, X, Bin, Wang, Ya-Bin, Zhang, Jian, Cao, Yu-Kang, Wu, Qing, Cao, Feng
• Format: Article
• Language: English
• Subjects: Adult; Age of Onset; Animals; bcl-2-Associated X Protein - metabolism; Brain Ischemia - diagnosis; Brain Ischemia - genetics; Brain Ischemia - metabolism; Case-Control Studies; Cell Line; Endothelial Cells - metabolism; Female; Genetic Association Studies; Genetic Predisposition to Disease; Heterozygote; Homozygote; Humans; Interleukin-1beta - genetics; Interleukin-1beta - metabolism; Male; Matrix Metalloproteinase 2 - metabolism; Mice; Myocardial Infarction - diagnosis; Myocardial Infarction - genetics; Myocardial Infarction - metabolism; NF-kappa B - genetics; NF-kappa B - metabolism; Nitric Oxide Synthase Type II - genetics; Nitric Oxide Synthase Type II - metabolism; Original; Phenotype; Polymorphism, Single Nucleotide; Rats; Risk Factors; Stroke - diagnosis; Stroke - genetics; Stroke - metabolism; Transfection
• ISSN: 1936-2625

In this study, by using vivo and vitro model, we assessed whether interleukin (IL)-1beta gene polymorphisms influence on the risk of myocardial infarction and ischemic stroke at young age. 147 patients (age < 45 years) with a first episode of MI and 56 patients (age < 45 years) with first-ever cerebral ischemia consecutively were admitted to this study from the Department of Chinese PLA General Hospital. Meanwhile, 91 normal volunteers without MI or stroke were deeded as control group and greed to give blood samples for DNA analysis and biochemical measurements by written informed consent. IL-1β-511 wild type (WT, CC) and SNP (TT) were established and transfected into Rat myocardial H9c2 cell and Mouse brain endothelial bEND.3 cells. In Young Age MI or stroke patients, the IL-1β levels of patients with 511CC are higher than that of patients with 511TT. In our study, NF-κB miRNA, iNOS activity, NF-κB, iNOS and Bax protein expressions of MI-induced H9c2 cell or stroke-induced bEND.3 cells in IL-1β-511TT group were lower than those of IL-1β-511CC. Additionally, the protein expression of MMP-2 of MI-induced H9c2 cell or stroke-induced bEND.3 cells in IL-1β-511TT group were higher than that of IL-1β 511CC group. In conclusion, our data indicate that IL-1β-511TT/CC influence on the risk of myocardial infarction and ischemic stroke at young age through NF-κB, iNOS, MMP-2 and Bax.