hTERT mediates gastric cancer metastasis partially through the indirect targeting of ITGB1 by microRNA-29a

• Published in: Scientific reports 2016-02, Vol.6 (1), p.21955-21955, Article 21955
• Main Authors: He, Bing, Xiao, Yu-Feng, Tang, Bo, Wu, Yu-Yun, Hu, Chang-Jiang, Xie, Rui, Yang, Xin, Yu, Song-Tao, Dong, Hui, Zhao, Xiao-Yan, Li, Ji-Liang, Yang, Shi-Ming
• Format: Article
• Language: English
• Subjects: 38/109; 631/67; 631/67/1504/1829; 64/60; Aged; Animals; Base Sequence; Cell Line, Tumor; Cell Movement; Epithelial Cells - metabolism; Epithelial Cells - pathology; Female; Gastric cancer; Gene Expression Regulation, Neoplastic; Genetic Vectors - chemistry; Genetic Vectors - metabolism; Humanities and Social Sciences; Humans; Injections, Subcutaneous; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - metabolism; Invasiveness; Lentivirus - genetics; Lentivirus - metabolism; Male; Membrane Proteins - genetics; Membrane Proteins - metabolism; Metastases; Metastasis; Mice; Mice, Nude; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; miRNA; multidisciplinary; Neoplasm Invasiveness; Neoplasm Metastasis; Neoplasm Transplantation; Osteoblasts - metabolism; Osteoblasts - pathology; RNA-directed DNA polymerase; Science; Science (multidisciplinary); Signal Transduction; Stomach Neoplasms - genetics; Stomach Neoplasms - mortality; Stomach Neoplasms - pathology; Stomach Neoplasms - surgery; Survival Analysis; Telomerase; Telomerase - genetics; Telomerase - metabolism; Telomerase reverse transcriptase
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep21955

Human telomerase reverse transcriptase (hTERT) plays a key role in tumor invasion and metastasis, but the mechanism of its involvement in these processes is not clear. The purpose of this study is to investigate the possible molecular mechanism of hTERT in the promotion of gastric cancer (GC) metastasis. We found that the up-regulation of hTERT in gastric cancer cells could inhibit the expression of miR-29a and enhance the expression of Integrin β1 (ITGB1). In addition, the invasive capacity of gastric cancer cells was also highly increased after hTERT overexpression. Our study also found that the restoration of miR-29a suppressed the expression of ITGB1 and inhibited GC cell metastasis both in vitro and in vivo . Taken together, our results suggested that hTERT may promote GC metastasis through the hTERT-miR-29a-ITGB1 regulatory pathway.