Bone-Healing Capacity of PCL/PLGA/Duck Beak Scaffold in Critical Bone Defects in a Rabbit Model

• Published in: BioMed research international 2016-01, Vol.2016 (2016), p.1-10
• Main Authors: Kang, Seong Soo, Son, Jun Sik, Son, Soo Jin, Lee, Jae Yeon, Choi, Seok Hwa
• Format: Article
• Language: English
• Subjects: Animals; Biocompatible Materials - chemistry; Biocompatible Materials - therapeutic use; Bone Regeneration; Bone Transplantation - methods; Calcium Phosphates - chemistry; Calcium Phosphates - therapeutic use; Defects; Fractures; Free radicals; Humans; Ischemia; Laboratory animals; Lactic Acid - chemistry; Lactic Acid - therapeutic use; Medical research; Osteogenesis - drug effects; Oxidative stress; Oxidative Stress - drug effects; Phosphatases; Phosphates; Polyesters - chemistry; Polyesters - therapeutic use; Polyglycolic Acid - chemistry; Polyglycolic Acid - therapeutic use; Rabbits; Studies; Tissue Engineering; Tissue Scaffolds - chemistry; Transmission Control Protocol/Internet Protocol (Computer network protocol); Veterinary colleges; Veterinary medicine
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2016/2136215

Bone defects are repaired using either natural or synthetic bone grafts. Poly( ϵ -caprolactone) (PCL), β-tricalcium phosphate (TCP), and poly(lactic-co-glycolic acid) (PLGA) are widely used as synthetic materials for tissue engineering. This study aimed to investigate the bone-healing capacity of PCL/PLGA/duck beak scaffold in critical bone defects and the oxidative stress status of the graft site in a rabbit model. The in vivo performance of 48 healthy New Zealand White rabbits, weighing between 2.5 and 3.5 kg, was evaluated. The rabbits were assigned to the following groups: group 1 (control), group 2 (PCL/PLGA hybrid scaffolds), group 3 (PCL/PLGA/TCP hybrid scaffolds), and group 4 (PCL/PLGA/DB hybrid scaffolds). A 5 mm critical defect was induced in the diaphysis of the left radius. X-ray, micro-CT, and histological analyses were conducted at (time 0) 4, 8, and 12 weeks after implantation. Furthermore, bone formation markers (bone-specific alkaline phosphatase, carboxyterminal propeptide of type I procollagen, and osteocalcin) were measured and oxidative stress status was determined. X-ray, micro-CT, biochemistry, and histological analyses revealed that the PCL/PLGA/duck beak scaffold promotes new bone formation in rabbit radius by inducing repair, suggesting that it could be a good option for the treatment of fracture.