Noninvasive Detection of Inflammatory Changes in White Adipose Tissue by Label-Free Raman Spectroscopy

White adipose tissue inflammation (WATi) has been linked to the pathogenesis of obesity-related diseases, including type 2 diabetes, cardiovascular disease, and cancer. In addition to the obese, a substantial number of normal and overweight individuals harbor WATi, putting them at increased risk for...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2016-02, Vol.88 (4), p.2140-2148
Main Authors: Haka, Abigail S, Sue, Erika, Zhang, Chi, Bhardwaj, Priya, Sterling, Joshua, Carpenter, Cassidy, Leonard, Madeline, Manzoor, Maryem, Walker, Jeanne, Aleman, Jose O, Gareau, Daniel, Holt, Peter R, Breslow, Jan L, Zhou, Xi Kathy, Giri, Dilip, Morrow, Monica, Iyengar, Neil, Barman, Ishan, Hudis, Clifford A, Dannenberg, Andrew J
Format: Article
Language:English
Subjects:
Adipose Tissue, White - pathology
Adipose tissues
Animals
Cancer
Cardiovascular disease
Diabetes
Disease Models, Animal
Diseases
Female
Humans
Inflammation - pathology
Male
Mice
Mice, Inbred C57BL
Obesity
Obesity - pathology
Pathogenesis
Raman spectroscopy
Risk
Risk assessment
Rodents
Spectrum Analysis, Raman - methods
Tissues
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Summary:White adipose tissue inflammation (WATi) has been linked to the pathogenesis of obesity-related diseases, including type 2 diabetes, cardiovascular disease, and cancer. In addition to the obese, a substantial number of normal and overweight individuals harbor WATi, putting them at increased risk for disease. We report the first technique that has the potential to detect WATi noninvasively. Here, we used Raman spectroscopy to detect WATi with excellent accuracy in both murine and human tissues. This is a potentially significant advance over current histopathological techniques for the detection of WATi, which rely on tissue excision and, therefore, are not practical for assessing disease risk in the absence of other identifying factors. Importantly, we show that noninvasive Raman spectroscopy can diagnose WATi in mice. Taken together, these results demonstrate the potential of Raman spectroscopy to provide objective risk assessment for future cardiometabolic complications in both normal weight and overweight/obese individuals.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.5b03696