A disulphide isomerase gene (PDI-V) from Haynaldia villosa contributes to powdery mildew resistance in common wheat

• Published in: Scientific reports 2016-04, Vol.6 (1), p.24227-24227, Article 24227
• Main Authors: Faheem, Muhammad, Li, Yingbo, Arshad, Muhammad, Jiangyue, Cheng, Jia, Zhao, Wang, Zongkuan, Xiao, Jin, Wang, Haiyan, Cao, Aizhong, Xing, Liping, Yu, Feifei, Zhang, Ruiqi, Xie, Qi, Wang, Xiue
• Format: Article
• Language: English
• Subjects: 13/44; 38/111; 38/77; 631/449/2491/2046; 631/449/711; Airborne microorganisms; Ascomycota - pathogenicity; Chromosomes, Plant - genetics; Cultivars; Disease Resistance - genetics; Endoplasmic reticulum; Endoplasmic Reticulum - metabolism; Gene silencing; Genes; Humanities and Social Sciences; multidisciplinary; Overexpression; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Poaceae - enzymology; Poaceae - genetics; Poaceae - immunology; Poaceae - microbiology; Point Mutation; Powdery mildew; Protein Disulfide-Isomerases - chemistry; Protein Disulfide-Isomerases - genetics; Protein Disulfide-Isomerases - metabolism; Protein Domains; Proteins; Science; Science (multidisciplinary); Seedlings; Thioredoxin; Triticum aestivum; Ubiquitin-protein ligase; Ubiquitination; V protein; Wheat
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep24227

In this study, we report the contribution of a PDI-like gene from wheat wild relative Haynaldia villosa in combating powdery mildew. PDI-V protein contains two conserved thioredoxin (TRX) active domains (a and a′) and an inactive domain (b). PDI-V interacted with E3 ligase CMPG1-V protein, which is a positive regulator of powdery mildew response. PDI-V was mono-ubiquitinated by CMPG1-V without degradation being detected. PDI-V was located on H. villosa chromosome 5V and encoded for a protein located in the endoplasmic reticulum. Bgt infection in leaves of H. villosa induced PDI-V expression. Virus induced gene silencing of PDIs in a T. durum-H. villosa amphiploid compromised the resistance. Single cell transient over-expression of PDI-V or a truncated version containing the active TXR domain a decreased the haustorial index in moderately susceptible wheat cultivar Yangmai 158. Stable transgenic lines over-expressing PDI-V in Yangmai 158 displayed improved powdery mildew resistance at both the seedling and adult stages. By contrast over-expression of point-mutated PDI-V C57A did not increase the level of resistance in Yangmai 158. The above results indicate a pivotal role of PDI-V in powdery mildew resistance and showed that conserved TRX domain a is critical for its function.