Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro

Epithelial-mesenchymal transition (EMT) induces the progression of renal tubulointerstitial fibrosis. Astragalus membranaceus (AM) is a traditional Chinese herbal medicine that has been demonstrated to exert anti-inflammatory and anti-cancer effects, in addition to protecting and supporting the immu...

Full description

Saved in:
Bibliographic Details
Published in:Experimental and therapeutic medicine 2016-05, Vol.11 (5), p.1611-1616
Main Authors: SHAN, GUANG, ZHOU, XIANG-JUN, XIA, YUE, QIAN, HUI-JUN
Format: Article
Language:English
Subjects:
Astragalus (Plants)
Astragalus membranaceus
Bioengineering
Biotechnology
Care and treatment
Cellular signal transduction
Chinese medicine
Cytokines
epithelial-mesenchymal transition
Fibroblasts
Fibrosis
Gene expression
Genetic aspects
Health aspects
Kidney diseases
Kinases
Phosphorylation
Polyclonal antibodies
Proteins
renal fibrosis
Studies
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c512t-1a0f5295ed16521e0104d4071793172ab85eb0d7e02cb3d92a0bde856e47918a3
cites cdi_FETCH-LOGICAL-c512t-1a0f5295ed16521e0104d4071793172ab85eb0d7e02cb3d92a0bde856e47918a3
container_end_page 1616
container_issue 5
container_start_page 1611
container_title Experimental and therapeutic medicine
container_volume 11
creator SHAN, GUANG
ZHOU, XIANG-JUN
XIA, YUE
QIAN, HUI-JUN
description Epithelial-mesenchymal transition (EMT) induces the progression of renal tubulointerstitial fibrosis. Astragalus membranaceus (AM) is a traditional Chinese herbal medicine that has been demonstrated to exert anti-inflammatory and anti-cancer effects, in addition to protecting and supporting the immune system. The present study investigated the effects of AM on renal fibrosis. A mouse model of unilateral ureteral obstruction (UUO) was established and treated with various concentrations of AM (100, 200 or 400 mg/kg/day). Interstitial fibrosis markedly increased in the UUO mice. AM significantly reduced the obstruction-induced upregulation of α-smooth muscle actin (α-SMA) and downregulation of E-cadherin in the kidneys of the UUO mice (P
doi_str_mv 10.3892/etm.2016.3152
format article
fullrecord <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4840494</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A453915869</galeid><sourcerecordid>A453915869</sourcerecordid><originalsourceid>FETCH-LOGICAL-c512t-1a0f5295ed16521e0104d4071793172ab85eb0d7e02cb3d92a0bde856e47918a3</originalsourceid><addsrcrecordid>eNptkk1v3CAQhq2qVROlOfZaWaoq9eINYGPjS6VVlH5IiXJJzwjMeJfIwBbwSvsf-qM7291sm6pwYIBnXj7mLYq3lCxq0bMryG7BCG0XNeXsRXFOu55VlFD-8hiTXtCz4jKlR4KNt1QI_ro4Yx1tRSfIefFzmXJUKzXNqXTgdFReDYAT5WCyIaoMqYzg1VRanyGmbLPFyWh1DMmmUu9wY201LvtVmWc9TyqWsLF5jQJqqhwk8MN65zALj_IJyeAxqdzabSiVN4c4x_CmeDWqKcHlcbwovn--ebj-Wt3ef_l2vbytBk5ZrqgiI2c9B0NbzigQShrTkA5fXNOOKS04aGI6IGzQtemZItqA4C00XU-Fqi-KTwfdzawdmAE8XmySm2idijsZlJXPd7xdy1XYykY0pOkbFPh4FIjhxwwpS2fTANOkPIQ5SSpY23aCkQ7R9_-gj2GO-J9I4XVJjWUSfyisBEjrx4DnDntRuWx43VMu2h6pxX8o7AacHYKH0eL6s4TqkDBgsVKE8fRGSuTeQRIdJPcOknsHIf_u74850U9-QeDDAUgbLJw1IZ2Ym4e7imD_LfQLlF3QHA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1931037928</pqid></control><display><type>article</type><title>Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro</title><source>Open Access: PubMed Central</source><creator>SHAN, GUANG ; ZHOU, XIANG-JUN ; XIA, YUE ; QIAN, HUI-JUN</creator><creatorcontrib>SHAN, GUANG ; ZHOU, XIANG-JUN ; XIA, YUE ; QIAN, HUI-JUN</creatorcontrib><description>Epithelial-mesenchymal transition (EMT) induces the progression of renal tubulointerstitial fibrosis. Astragalus membranaceus (AM) is a traditional Chinese herbal medicine that has been demonstrated to exert anti-inflammatory and anti-cancer effects, in addition to protecting and supporting the immune system. The present study investigated the effects of AM on renal fibrosis. A mouse model of unilateral ureteral obstruction (UUO) was established and treated with various concentrations of AM (100, 200 or 400 mg/kg/day). Interstitial fibrosis markedly increased in the UUO mice. AM significantly reduced the obstruction-induced upregulation of α-smooth muscle actin (α-SMA) and downregulation of E-cadherin in the kidneys of the UUO mice (P&lt;0.05). Furthermore, AM treatment significantly inhibited the induction of EMT and the deposition of extracellular matrix. In addition, a transforming growth factor (TGF)-β1-stimulated murine renal proximal tubule cell line (NRK-52E) was treated with various concentrations of AM (10, 20, and 40 µg/ml). E-cadherin expression levels significantly decreased and those of α-SMA significantly increased in NRK-52E cells stimulated with TGF-β1 in vitro (P&lt;0.05). Co-treatment with AM reversed these effects (P&lt;0.05), and AM treatment reduced TGF-β1-induced expression and Smad2/3 phosphorylation (P&lt;0.05). These results suggested that AM antagonizes tubular EMT by inhibiting the Smad signaling pathway.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2016.3152</identifier><identifier>PMID: 27168780</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Astragalus (Plants) ; Astragalus membranaceus ; Bioengineering ; Biotechnology ; Care and treatment ; Cellular signal transduction ; Chinese medicine ; Cytokines ; epithelial-mesenchymal transition ; Fibroblasts ; Fibrosis ; Gene expression ; Genetic aspects ; Health aspects ; Kidney diseases ; Kinases ; Phosphorylation ; Polyclonal antibodies ; Proteins ; renal fibrosis ; Studies</subject><ispartof>Experimental and therapeutic medicine, 2016-05, Vol.11 (5), p.1611-1616</ispartof><rights>Copyright: © Shan et al.</rights><rights>COPYRIGHT 2016 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2016</rights><rights>Copyright: © Shan et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-1a0f5295ed16521e0104d4071793172ab85eb0d7e02cb3d92a0bde856e47918a3</citedby><cites>FETCH-LOGICAL-c512t-1a0f5295ed16521e0104d4071793172ab85eb0d7e02cb3d92a0bde856e47918a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840494/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840494/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27168780$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SHAN, GUANG</creatorcontrib><creatorcontrib>ZHOU, XIANG-JUN</creatorcontrib><creatorcontrib>XIA, YUE</creatorcontrib><creatorcontrib>QIAN, HUI-JUN</creatorcontrib><title>Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>Epithelial-mesenchymal transition (EMT) induces the progression of renal tubulointerstitial fibrosis. Astragalus membranaceus (AM) is a traditional Chinese herbal medicine that has been demonstrated to exert anti-inflammatory and anti-cancer effects, in addition to protecting and supporting the immune system. The present study investigated the effects of AM on renal fibrosis. A mouse model of unilateral ureteral obstruction (UUO) was established and treated with various concentrations of AM (100, 200 or 400 mg/kg/day). Interstitial fibrosis markedly increased in the UUO mice. AM significantly reduced the obstruction-induced upregulation of α-smooth muscle actin (α-SMA) and downregulation of E-cadherin in the kidneys of the UUO mice (P&lt;0.05). Furthermore, AM treatment significantly inhibited the induction of EMT and the deposition of extracellular matrix. In addition, a transforming growth factor (TGF)-β1-stimulated murine renal proximal tubule cell line (NRK-52E) was treated with various concentrations of AM (10, 20, and 40 µg/ml). E-cadherin expression levels significantly decreased and those of α-SMA significantly increased in NRK-52E cells stimulated with TGF-β1 in vitro (P&lt;0.05). Co-treatment with AM reversed these effects (P&lt;0.05), and AM treatment reduced TGF-β1-induced expression and Smad2/3 phosphorylation (P&lt;0.05). These results suggested that AM antagonizes tubular EMT by inhibiting the Smad signaling pathway.</description><subject>Astragalus (Plants)</subject><subject>Astragalus membranaceus</subject><subject>Bioengineering</subject><subject>Biotechnology</subject><subject>Care and treatment</subject><subject>Cellular signal transduction</subject><subject>Chinese medicine</subject><subject>Cytokines</subject><subject>epithelial-mesenchymal transition</subject><subject>Fibroblasts</subject><subject>Fibrosis</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Kidney diseases</subject><subject>Kinases</subject><subject>Phosphorylation</subject><subject>Polyclonal antibodies</subject><subject>Proteins</subject><subject>renal fibrosis</subject><subject>Studies</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNptkk1v3CAQhq2qVROlOfZaWaoq9eINYGPjS6VVlH5IiXJJzwjMeJfIwBbwSvsf-qM7291sm6pwYIBnXj7mLYq3lCxq0bMryG7BCG0XNeXsRXFOu55VlFD-8hiTXtCz4jKlR4KNt1QI_ro4Yx1tRSfIefFzmXJUKzXNqXTgdFReDYAT5WCyIaoMqYzg1VRanyGmbLPFyWh1DMmmUu9wY201LvtVmWc9TyqWsLF5jQJqqhwk8MN65zALj_IJyeAxqdzabSiVN4c4x_CmeDWqKcHlcbwovn--ebj-Wt3ef_l2vbytBk5ZrqgiI2c9B0NbzigQShrTkA5fXNOOKS04aGI6IGzQtemZItqA4C00XU-Fqi-KTwfdzawdmAE8XmySm2idijsZlJXPd7xdy1XYykY0pOkbFPh4FIjhxwwpS2fTANOkPIQ5SSpY23aCkQ7R9_-gj2GO-J9I4XVJjWUSfyisBEjrx4DnDntRuWx43VMu2h6pxX8o7AacHYKH0eL6s4TqkDBgsVKE8fRGSuTeQRIdJPcOknsHIf_u74850U9-QeDDAUgbLJw1IZ2Ym4e7imD_LfQLlF3QHA</recordid><startdate>20160501</startdate><enddate>20160501</enddate><creator>SHAN, GUANG</creator><creator>ZHOU, XIANG-JUN</creator><creator>XIA, YUE</creator><creator>QIAN, HUI-JUN</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160501</creationdate><title>Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro</title><author>SHAN, GUANG ; ZHOU, XIANG-JUN ; XIA, YUE ; QIAN, HUI-JUN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-1a0f5295ed16521e0104d4071793172ab85eb0d7e02cb3d92a0bde856e47918a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Astragalus (Plants)</topic><topic>Astragalus membranaceus</topic><topic>Bioengineering</topic><topic>Biotechnology</topic><topic>Care and treatment</topic><topic>Cellular signal transduction</topic><topic>Chinese medicine</topic><topic>Cytokines</topic><topic>epithelial-mesenchymal transition</topic><topic>Fibroblasts</topic><topic>Fibrosis</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Kidney diseases</topic><topic>Kinases</topic><topic>Phosphorylation</topic><topic>Polyclonal antibodies</topic><topic>Proteins</topic><topic>renal fibrosis</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SHAN, GUANG</creatorcontrib><creatorcontrib>ZHOU, XIANG-JUN</creatorcontrib><creatorcontrib>XIA, YUE</creatorcontrib><creatorcontrib>QIAN, HUI-JUN</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SHAN, GUANG</au><au>ZHOU, XIANG-JUN</au><au>XIA, YUE</au><au>QIAN, HUI-JUN</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro</atitle><jtitle>Experimental and therapeutic medicine</jtitle><addtitle>Exp Ther Med</addtitle><date>2016-05-01</date><risdate>2016</risdate><volume>11</volume><issue>5</issue><spage>1611</spage><epage>1616</epage><pages>1611-1616</pages><issn>1792-0981</issn><eissn>1792-1015</eissn><abstract>Epithelial-mesenchymal transition (EMT) induces the progression of renal tubulointerstitial fibrosis. Astragalus membranaceus (AM) is a traditional Chinese herbal medicine that has been demonstrated to exert anti-inflammatory and anti-cancer effects, in addition to protecting and supporting the immune system. The present study investigated the effects of AM on renal fibrosis. A mouse model of unilateral ureteral obstruction (UUO) was established and treated with various concentrations of AM (100, 200 or 400 mg/kg/day). Interstitial fibrosis markedly increased in the UUO mice. AM significantly reduced the obstruction-induced upregulation of α-smooth muscle actin (α-SMA) and downregulation of E-cadherin in the kidneys of the UUO mice (P&lt;0.05). Furthermore, AM treatment significantly inhibited the induction of EMT and the deposition of extracellular matrix. In addition, a transforming growth factor (TGF)-β1-stimulated murine renal proximal tubule cell line (NRK-52E) was treated with various concentrations of AM (10, 20, and 40 µg/ml). E-cadherin expression levels significantly decreased and those of α-SMA significantly increased in NRK-52E cells stimulated with TGF-β1 in vitro (P&lt;0.05). Co-treatment with AM reversed these effects (P&lt;0.05), and AM treatment reduced TGF-β1-induced expression and Smad2/3 phosphorylation (P&lt;0.05). These results suggested that AM antagonizes tubular EMT by inhibiting the Smad signaling pathway.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>27168780</pmid><doi>10.3892/etm.2016.3152</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1792-0981
ispartof Experimental and therapeutic medicine, 2016-05, Vol.11 (5), p.1611-1616
issn 1792-0981
1792-1015
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4840494
source Open Access: PubMed Central
subjects Astragalus (Plants)
Astragalus membranaceus
Bioengineering
Biotechnology
Care and treatment
Cellular signal transduction
Chinese medicine
Cytokines
epithelial-mesenchymal transition
Fibroblasts
Fibrosis
Gene expression
Genetic aspects
Health aspects
Kidney diseases
Kinases
Phosphorylation
Polyclonal antibodies
Proteins
renal fibrosis
Studies
title Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T02%3A57%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Astragalus%20membranaceus%20ameliorates%20renal%20interstitial%20fibrosis%20by%20inhibiting%20tubular%20epithelial-mesenchymal%20transition%20in%20vivo%20and%20in%20vitro&rft.jtitle=Experimental%20and%20therapeutic%20medicine&rft.au=SHAN,%20GUANG&rft.date=2016-05-01&rft.volume=11&rft.issue=5&rft.spage=1611&rft.epage=1616&rft.pages=1611-1616&rft.issn=1792-0981&rft.eissn=1792-1015&rft_id=info:doi/10.3892/etm.2016.3152&rft_dat=%3Cgale_pubme%3EA453915869%3C/gale_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c512t-1a0f5295ed16521e0104d4071793172ab85eb0d7e02cb3d92a0bde856e47918a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1931037928&rft_id=info:pmid/27168780&rft_galeid=A453915869&rfr_iscdi=true