Skeletal muscle satellite cells are located at a closer proximity to capillaries in healthy young compared with older men

Background Skeletal muscle satellite cells (SC) are instrumental in maintenance of muscle fibres, the adaptive responses to exercise, and there is an age‐related decline in SC. A spatial relationship exists between SC and muscle fibre capillaries. In the present study, we aimed to investigate whethe...

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Bibliographic Details
Published in:Journal of cachexia, sarcopenia and muscle sarcopenia and muscle, 2016-12, Vol.7 (5), p.547-554
Main Authors: Nederveen, Joshua P, Joanisse, Sophie, Snijders, Tim, Ivankovic, Victoria, Baker, Steven K., Phillips, Stuart M., Parise, Gianni
Format: Article
Language:English
Subjects:
Adult
Age Factors
Aged
Aging
Biomarkers
Biopsy
Capillaries
Capillaries - metabolism
Cell Count
Cellular Microenvironment
Exercise
Fitness training programs
Fluorescent Antibody Technique
Growth factors
Healthy Volunteers
Humans
Immunoglobulins
Male
Middle Aged
Muscle Fibers, Skeletal - cytology
Muscle Fibers, Skeletal - metabolism
Muscle stem cells
Muscle, Skeletal - blood supply
Muscle, Skeletal - cytology
Muscle, Skeletal - metabolism
Musculoskeletal system
MyoD
Older people
Original
Pax7
Perfusion
Physical fitness
Satellite Cells, Skeletal Muscle - cytology
Satellite Cells, Skeletal Muscle - metabolism
Weightlifting
Young Adult
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Summary:Background Skeletal muscle satellite cells (SC) are instrumental in maintenance of muscle fibres, the adaptive responses to exercise, and there is an age‐related decline in SC. A spatial relationship exists between SC and muscle fibre capillaries. In the present study, we aimed to investigate whether chronologic age has an impact on the spatial relationship between SC and muscle fibre capillaries. Secondly, we determined whether this spatial relationship changes in response to a single session of resistance exercise. Methods Muscle biopsies were obtained from the vastus lateralis of previously untrained young men (YM, 24 ± 3 years; n = 23) and older men (OM, 67 ± 4 years; n = 22) at rest. A subset of YM (n = 9) performed a single bout of resistance exercise, where additional muscle biopsies taken at 24 and 72 h post‐exercise recovery. Skeletal muscle fibre capillarization, SC content, and activation status were assessed using immunofluorescent microscopy of muscle cross sections. Results Type II muscle fibre SC and capillary content was significantly lower in the YM compared with OM (P 
ISSN:2190-5991
2190-6009
DOI:10.1002/jcsm.12105