The long noncoding RNA CASC2 functions as a competing endogenous RNA by sponging miR-18a in colorectal cancer

Recent evidence highlights the crucial regulatory roles of long noncoding RNAs (lncRNA) in tumor biology. In colorectal cancer (CRC), the expression of several lncRNAs is dysregulated and play essential roles in CRC tumorigenesis. However, the potential biological roles and regulatory mechanisms of...

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Bibliographic Details
Published in:Scientific reports 2016-05, Vol.6 (1), p.26524-26524, Article 26524
Main Authors: Huang, Guanli, Wu, Xiaoli, Li, Shi, Xu, Xiaoqun, Zhu, Hua, Chen, Xiangjian
Format: Article
Language:English
Subjects:
13/1
13/106
38/89
631/67/1504/1885/1393
692/4028/67/1857
Cell proliferation
Colorectal cancer
Colorectal carcinoma
Gene silencing
Humanities and Social Sciences
Metastases
multidisciplinary
Phase transitions
Ribonucleic acid
RNA
S phase
Science
Science (multidisciplinary)
Stat3 protein
Tumorigenesis
Tumors
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Summary:Recent evidence highlights the crucial regulatory roles of long noncoding RNAs (lncRNA) in tumor biology. In colorectal cancer (CRC), the expression of several lncRNAs is dysregulated and play essential roles in CRC tumorigenesis. However, the potential biological roles and regulatory mechanisms of the novel human lncRNA, CASC2 (cancer susceptibility candidate 2), in tumor biology are poorly understood. In this study, CASC2 expression was significantly decreased in CRC tissues and CRC cell lines, and decreased expression was significantly more frequent in patients with advanced tumor-node-metastasis stage disease (TNM III and IV) ( P  = 0.028). Further functional experiments indicate that CASC2 could directly upregulate PIAS3 expression by functioning as a competing endogenous RNA (ceRNA) for miR-18a. This interactions leads to the de-repression of genes downstream of STAT3 and consequentially inhibition of CRC cell proliferation and tumor growth in vitro and in vivo by extending the G 0 /G 1 -S phase transition. Taken together, these observations suggest CASC2 as a ceRNA plays an important role in CRC pathogenesis and may serve as a potential target for cancer diagnosis and treatment.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep26524