Folding complex DNA nanostructures from limited sets of reusable sequences

Scalable production of DNA nanostructures remains a substantial obstacle to realizing new applications of DNA nanotechnology. Typical DNA nanostructures comprise hundreds of DNA oligonucleotide strands, where each unique strand requires a separate synthesis step. New design methods that reduce the s...

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Bibliographic Details
Published in:Nucleic acids research 2016-06, Vol.44 (11), p.e102-e102
Main Authors: Niekamp, Stefan, Blumer, Katy, Nafisi, Parsa M, Tsui, Kathy, Garbutt, John, Douglas, Shawn M
Format: Article
Language:English
Subjects:
Base Sequence
DNA - chemistry
Methods Online
Nanostructures
Nanotechnology
Nucleic Acid Conformation
Oligonucleotides - chemistry
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Summary:Scalable production of DNA nanostructures remains a substantial obstacle to realizing new applications of DNA nanotechnology. Typical DNA nanostructures comprise hundreds of DNA oligonucleotide strands, where each unique strand requires a separate synthesis step. New design methods that reduce the strand count for a given shape while maintaining overall size and complexity would be highly beneficial for efficiently producing DNA nanostructures. Here, we report a method for folding a custom template strand by binding individual staple sequences to multiple locations on the template. We built several nanostructures for well-controlled testing of various design rules, and demonstrate folding of a 6-kb template by as few as 10 unique strand sequences binding to 10 ± 2 locations on the template strand.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkw208