Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Fails To Identify Nontuberculous Mycobacteria from Primary Cultures of Respiratory Samples

We have assessed matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identification (Bruker) of nontuberculous mycobacteria from newly positive liquid cultures of respiratory samples. Twelve (22%) of 54 isolates were identified directly from liquid medium. Aft...

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Bibliographic Details
Published in:Journal of clinical microbiology 2016-07, Vol.54 (7), p.1915-1917
Main Authors: van Eck, Kim, Faro, Dirk, Wattenberg, Melanie, de Jong, Arjan, Kuipers, Saskia, van Ingen, Jakko
Format: Article
Language:English
Subjects:
Bacteriological Techniques - methods
Humans
Mycobacteriology and Aerobic Actinomycetes
Mycobacterium Infections, Nontuberculous - diagnosis
Mycobacterium Infections, Nontuberculous - microbiology
Nontuberculous Mycobacteria - chemistry
Nontuberculous Mycobacteria - isolation & purification
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Sputum - microbiology
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Summary:We have assessed matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identification (Bruker) of nontuberculous mycobacteria from newly positive liquid cultures of respiratory samples. Twelve (22%) of 54 isolates were identified directly from liquid medium. After subculture and with manual laser operation, this rose to 49/54 isolates (91%). MALDI-TOF MS is less promising than previously suggested.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00304-16