Residualization Rates of Near-Infrared Dyes for the Rational Design of Molecular Imaging Agents

Purpose Near-infrared (NIR) fluorescence imaging is widely used for tracking antibodies and biomolecules in vivo . Clinical and preclinical applications include intraoperative imaging, tracking therapeutics, and fluorescent labeling as a surrogate for subsequent radiolabeling. Despite their extensiv...

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Bibliographic Details
Published in:Molecular imaging and biology 2015-12, Vol.17 (6), p.757-762
Main Authors: Cilliers, Cornelius, Liao, Jianshan, Atangcho, Lydia, Thurber, Greg M.
Format: Article
Language:English
Subjects:
Brief Article
Cell Line
Fluorescent Dyes - chemistry
Humans
Imaging
Medicine
Medicine & Public Health
Microscopy, Confocal
Molecular Imaging - methods
Radiology
Spectroscopy, Near-Infrared
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Summary:Purpose Near-infrared (NIR) fluorescence imaging is widely used for tracking antibodies and biomolecules in vivo . Clinical and preclinical applications include intraoperative imaging, tracking therapeutics, and fluorescent labeling as a surrogate for subsequent radiolabeling. Despite their extensive use, one of the fundamental properties of NIR dyes, the residualization rate within cells following internalization, has not been systematically studied. This rate is required for the rational design of probes and proper interpretation of in vivo results. Procedures In this brief report, we measure the cellular residualization rate of eight commonly used dyes encompassing three core structures (cyanine, boron-dipyrromethene (BODIPY), and oxazine/thiazine/carbopyronin). Results We identify residualizing (half-life >24 h) and non-residualizing (half-life
ISSN:1536-1632
1860-2002
DOI:10.1007/s11307-015-0851-7