Components of chicken egg white extract smaller than 3 kDa in size promote 293T cell proliferation

We previously found that chicken egg white extract could promote cell survival and proliferation. In the present study, we further separated this extract into its components to identify those primarily responsible for promoting cell proliferation. Components of differing molecular weight were separa...

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Bibliographic Details
Published in:Cytotechnology (Dordrecht) 2016-08, Vol.68 (4), p.1115-1122
Main Authors: Ruan, Guang-ping, Yao, Xiang, Wang, Jin-xiang, Liu, Ju-fen, Shu, Fan, Li, Zi-an, Pang, Rong-qing, Pan, Xing-hua
Format: Article
Language:English
Subjects:
Albumen
Biochemistry
Biological activity
Biomedicine
Biotechnology
Cell culture
Cell growth
Cell proliferation
Cell size
Cell survival
Chemistry
Chemistry and Materials Science
Culture media
Eggs
Kinases
Molecular weight
Original Research
Paternity
Proteins
Ultrafiltration
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Summary:We previously found that chicken egg white extract could promote cell survival and proliferation. In the present study, we further separated this extract into its components to identify those primarily responsible for promoting cell proliferation. Components of differing molecular weight were separated from chicken egg white extract by ultrafiltration and 293T cell cultures were supplemented with various concentrations. The effects on cell proliferation were subsequently determined by a CellTiter 96 Aqueous One Solution Cell Proliferation Assay kit (Promega). We demonstrate that components from chicken egg white smaller than 3 kDa in size are able to function as active ingredients promoting cellular proliferation. This discovery may identify a new and convenient additive for cell culture media to promote cell growth and proliferation.
ISSN:0920-9069
1573-0778
DOI:10.1007/s10616-015-9868-7