Effects of intrinsic aerobic capacity and ovariectomy on voluntary wheel running and nucleus accumbens dopamine receptor gene expression

• Published in: Physiology & behavior 2016-10, Vol.164 (Pt A), p.383-389
• Main Authors: Park, Young-Min, Kanaley, Jill A, Padilla, Jaume, Zidon, Terese, Welly, Rebecca J, Will, Matthew J, Britton, Steven L, Koch, Lauren G, Ruegsegger, Gregory N, Booth, Frank W, Thyfault, John P, Vieira-Potter, Victoria J
• Format: Article
• Language: English
• Subjects: Aerobic capacity; Animals; Dopamine Plasma Membrane Transport Proteins - metabolism; Dopamine receptors; Exercise motivation; Female; Gene Expression - physiology; Models, Animal; Motivation - physiology; Motor Activity - physiology; Nucleus accumbens; Nucleus Accumbens - metabolism; Ovariectomy; Ovariectomy - adverse effects; Ovariectomy - psychology; Physical Fitness - physiology; Physical Fitness - psychology; Psychiatry; Random Allocation; Rats; Receptors, Dopamine - metabolism; RNA, Messenger - metabolism; Running - physiology; Running - psychology; Species Specificity; Volition - physiology
• ISSN: 0031-9384; 1873-507X
• DOI: 10.1016/j.physbeh.2016.06.006

Abstract Rats selectively bred for high (HCR) and low (LCR) aerobic capacity show a stark divergence in wheel running behavior, which may be associated with the dopamine (DA) system in the brain. HCR possess greater motivation for voluntary running along with greater brain DA activity compared to LCR. We recently demonstrated that HCR are not immune to ovariectomy (OVX)-associated reductions in spontaneous cage (i.e. locomotor) activity. Whether HCR and LCR rats differ in their OVX-mediated voluntary wheel running response is unknown. Purpose To determine whether HCR are protected from OVX-associated reduction in voluntary wheel running. Methods Forty female HCR and LCR rats (age ~ 27 weeks) had either SHM or OVX operations, and given access to a running wheel for 11 weeks. Weekly wheel running distance was monitored throughout the intervention. Nucleus accumbens (NAc) was assessed for mRNA expression of DA receptors at sacrifice. Results Compared to LCR, HCR ran greater distance and had greater ratio of excitatory/inhibitory DA mRNA expression (both line main effects, P < 0.05). Wheel running distance was significantly, positively correlated with the ratio of excitatory/inhibitory DA mRNA expression across animals. In both lines, OVX reduced wheel running ( P < 0.05). Unexpectedly, although HCR started with significantly greater voluntary wheel running, they had greater OVX-induced reduction in wheel running than LCR such that no differences were found 11 weeks after OVX between HCROVX and LCROVX (interaction, P < 0.05). This significant reduction in wheel running in HCR was associated with an OVX-mediated reduction in the ratio of excitatory/inhibitory DA mRNA expression. Conclusion The DA system in the NAc region may play a significant role in motivation to run in female rats. Compared to LCR, HCR rats run significantly more, which associates with greater ratio of excitatory/inhibitory DA mRNA expression. However, despite greater inherent motivation to run and an associated brain DA mRNA expression profile, HCR rats are not protected against OVX-induced reduction in wheel running or OVX-mediated reduction in the ratio of excitatory/inhibitory DA receptor mRNA expression. OVX-mediated reduction in motivated physical activity may be partially explained by a reduced ratio of excitatory/inhibitory DA receptor mRNA expression for which intrinsic fitness does not confer protection.