Paediatric rhabdomyosarcoma: MyoD1 demonstration in routinely processed tissue sections using wet heat pretreatment (pressure cooking) for antigen retrieval

AIMS: To investigate wet heat pretreatment (pressure cooking) as a means of antigen retrieval for demonstration of MyoD1 in paraffin wax embedded tissue. METHODS: Routinely processed tissue sections of transmission electron microscope confirmed cases of rhabdomyosarcoma were stained immunohistochemi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of clinical pathology 1997-01, Vol.50 (1), p.37-39
Main Authors: Engel, M E, Mouton, S C, Emms, M
Format: Article
Language:English
Subjects:
Biological and medical sciences
Histocytological Preparation Techniques
Humans
Immunohistochemistry
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Miscellaneous. Technology
MyoD Protein - metabolism
Neoplasm Proteins - metabolism
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Rhabdomyosarcoma - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:AIMS: To investigate wet heat pretreatment (pressure cooking) as a means of antigen retrieval for demonstration of MyoD1 in paraffin wax embedded tissue. METHODS: Routinely processed tissue sections of transmission electron microscope confirmed cases of rhabdomyosarcoma were stained immunohistochemically with the MyoD1 antibody. Antigen retrieval was achieved by wet heat pretreatment of the tissue sections. RESULTS: MyoD1 was stained successfully in all seven cases. The protein was localised to nuclei and cytoplasm depending on the type of tumour cell. CONCLUSIONS: Wet heat pretreatment for antigen retrieval from routinely processed tissue sections permits excellent subsequent immunostaining for MyoD1 in rhabdomyoblasts.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.50.1.37