Spatiotemporal Fluctuation Analysis: A Powerful Tool for the Future Nanoscopy of Molecular Processes

The enormous wealth of information available today from optical microscopy measurements on living samples is often underexploited. We argue that spatiotemporal analysis of fluorescence fluctuations using multiple detection channels can enhance the performance of current nanoscopy methods and provide...

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Bibliographic Details
Published in:Biophysical journal 2016-08, Vol.111 (4), p.679-685
Main Authors: Di Rienzo, Carmine, Gratton, Enrico, Beltram, Fabio, Cardarelli, Francesco
Format: Article
Language:English
Subjects:
Biophysical
Fluorescence
Image Processing, Computer-Assisted
Microscopy
Microscopy, Fluorescence - methods
Molecular biology
Nanostructured materials
Nanotechnology - methods
Spatio-Temporal Analysis
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Summary:The enormous wealth of information available today from optical microscopy measurements on living samples is often underexploited. We argue that spatiotemporal analysis of fluorescence fluctuations using multiple detection channels can enhance the performance of current nanoscopy methods and provide further insight into dynamic molecular processes of high biological relevance.
ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2016.07.015