Aberration-free volumetric high-speed imaging of in vivo retina

Certain topics in research and advancements in medical diagnostics may benefit from improved temporal and spatial resolution during non-invasive optical imaging of living tissue. However, so far no imaging technique can generate entirely diffraction-limited tomographic volumes with a single data acq...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2016-10, Vol.6 (1), p.35209-35209, Article 35209
Main Authors: Hillmann, Dierck, Spahr, Hendrik, Hain, Carola, Sudkamp, Helge, Franke, Gesa, Pfäffle, Clara, Winter, Christian, Hüttmann, Gereon
Format: Article
Language:English
Subjects:
631/1647/245/2221
631/1647/245/2226
639/624/1075/1076
Capillaries - anatomy & histology
Capillaries - ultrastructure
Data acquisition
Diffraction
Healthy Volunteers
Humanities and Social Sciences
Humans
Image Processing, Computer-Assisted - statistics & numerical data
Imaging, Three-Dimensional - instrumentation
Imaging, Three-Dimensional - methods
Interferometry - instrumentation
Interferometry - methods
Medical imaging
multidisciplinary
Nerve Fibers - ultrastructure
Optical Imaging - instrumentation
Optical Imaging - methods
Retina
Retina - anatomy & histology
Retina - ultrastructure
Science
Spatial discrimination
Time Factors
Tomography, Optical Coherence - instrumentation
Tomography, Optical Coherence - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Certain topics in research and advancements in medical diagnostics may benefit from improved temporal and spatial resolution during non-invasive optical imaging of living tissue. However, so far no imaging technique can generate entirely diffraction-limited tomographic volumes with a single data acquisition, if the target moves or changes rapidly, such as the human retina. Additionally, the presence of aberrations may represent further difficulties. We show that a simple interferometric setup–based on parallelized optical coherence tomography–acquires volumetric data with 10 billion voxels per second, exceeding previous imaging speeds by an order of magnitude. This allows us to computationally obtain and correct defocus and aberrations resulting in entirely diffraction-limited volumes. As demonstration, we imaged living human retina with clearly visible nerve fiber layer, small capillary networks, and photoreceptor cells. Furthermore, the technique can also obtain phase-sensitive volumes of other scattering structures at unprecedented acquisition speeds.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep35209