Conditioning with Fludarabine-Busulfan versus Busulfan-Cyclophosphamide Is Associated with Lower aGVHD and Higher Survival but More Extensive and Long Standing Bone Marrow Damage

Acute graft-versus-host disease (aGVHD) is a major complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT) and a major cause of nonrelapse mortality after allo-HSCT. A conditioning regimen plays a pivotal role in the development of aGVHD. To provide a platform for studying aGV...

Full description

Saved in:
Bibliographic Details
Published in:BioMed research international 2016-01, Vol.2016 (2016), p.1-10
Main Authors: Guo, KunYuan, Weng, Guangyang, Huang, YuXian, Wang, Jing, Xu, XiaoJun, Ye, YongBin, He, Xin, Zhang, MingWan
Format: Article
Language:English
Subjects:
Analysis
Animals
Bone marrow
Bone Marrow Cells - drug effects
Bone Marrow Transplantation
Busulfan
Busulfan - administration & dosage
Chemotherapy
Clinical medicine
Cyclophosphamide
Cyclophosphamide - administration & dosage
Cytokines
Disease Models, Animal
Drug Combinations
Gene expression
Gene Expression Regulation - drug effects
Graft vs Host Disease - drug therapy
Graft vs Host Disease - etiology
Graft vs Host Disease - immunology
Health aspects
Hematology
Hematopoietic Stem Cell Transplantation - adverse effects
Hematopoietic stem cells
Hospitals
Humans
Influenza
Interferon-gamma - biosynthesis
Interleukin-4 - blood
Leukemia
Lymphoma
Medical research
Mice
Mortality
Pathogenesis
Studies
T cells
T-Lymphocytes - drug effects
Transplantation
Transplantation, Homologous - adverse effects
Vidarabine - administration & dosage
Vidarabine - analogs & derivatives
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Acute graft-versus-host disease (aGVHD) is a major complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT) and a major cause of nonrelapse mortality after allo-HSCT. A conditioning regimen plays a pivotal role in the development of aGVHD. To provide a platform for studying aGVHD and evaluating the impact of different conditioning regimens, we established a murine aGVHD model that simulates the clinical situation and can be conditioned with Busulfan-Cyclophosphamide (Bu-Cy) and Fludarabine-Busulfan (Flu-Bu). In our study, BALB/c mice were conditioned with Bu-Cy or Flu-Bu and transplanted with 2×107 bone marrow cells and 2×107 splenocytes from either allogeneic (C57BL/6) or syngeneic (BALB/c) donors. The allogeneic recipients conditioned with Bu-Cy had shorter survivals (P
ISSN:2314-6133
2314-6141
DOI:10.1155/2016/3071214