H2-Fueled ATP Synthesis on an Electrode: Mimicking Cellular Respiration

ATP, the molecule used by living organisms to supply energy to many different metabolic processes, is synthesized mostly by the ATPase synthase using a proton or sodium gradient generated across a lipid membrane. We present evidence that a modified electrode surface integrating a NiFeSe hydrogenase...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2016-05, Vol.55 (21), p.6216-6220
Main Authors: Gutiérrez-Sanz, Óscar, Natale, Paolo, Márquez, Ileana, Marques, Marta C., Zacarias, Sonia, Pita, Marcos, Pereira, Inês A. C., López-Montero, Iván, De Lacey, Antonio L., Vélez, Marisela
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
ATP
bioelectrochemistry
biophysics
Chemical synthesis
Communication
Communications
Electrochemical oxidation
Electrochemical Techniques
Electrochemistry
Electrodes
Enzymatic synthesis
Hydrogen - chemistry
Hydrogen - metabolism
Hydrogenase
Hydrogenase - chemistry
Hydrogenase - metabolism
immobilization
Lipid Bilayers - chemistry
membrane proteins
Microscopy, Atomic Force
Mimicry
Mitochondrial Proton-Translocating ATPases - chemistry
Mitochondrial Proton-Translocating ATPases - metabolism
Molecular chains
Oxidation
Oxidation-Reduction
proton transport
Quartz Crystal Microbalance Techniques
Sodium
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Summary:ATP, the molecule used by living organisms to supply energy to many different metabolic processes, is synthesized mostly by the ATPase synthase using a proton or sodium gradient generated across a lipid membrane. We present evidence that a modified electrode surface integrating a NiFeSe hydrogenase and a F1F0‐ATPase in a lipid membrane can couple the electrochemical oxidation of H2 to the synthesis of ATP. This electrode‐assisted conversion of H2 gas into ATP could serve to generate this biochemical fuel locally when required in biomedical devices or enzymatic synthesis of valuable products. ATP synthesis fueled by H2: A gold electrode modified with an oriented membrane‐bound NiFeSe hydrogenase, a lipid membrane, and an integrated F1F0‐ATPase generates a local proton gradient by electroenzymatic H2 oxidation, which is then used to synthesize ATP. This electrode‐assisted conversion of H2 into ATP could locally generate biochemical fuel in medical devices or enzymatic synthesis.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201600752