Comprehensive analysis of the long noncoding RNA HOXA11-AS gene interaction regulatory network in NSCLC cells

• Published in: Cancer cell international 2016-12, Vol.16 (1), p.89-89, Article 89
• Main Authors: Zhang, Yu, He, Rong-Quan, Dang, Yi-Wu, Zhang, Xiu-Ling, Wang, Xiao, Huang, Su-Ning, Huang, Wen-Ting, Jiang, Meng-Tong, Gan, Xiao-Ning, Xie, You, Li, Ping, Luo, Dian-Zhong, Chen, Gang, Gan, Ting-Qing
• Format: Article
• Language: English
• Subjects: Hypothesis
• ISSN: 1475-2867; 1475-2867
• DOI: 10.1186/s12935-016-0366-6

Long noncoding RNAs (lncRNAs) are related to different biological processes in non-small cell lung cancer (NSCLC). However, the possible molecular mechanisms underlying the effects of the long noncoding RNA HOXA11-AS (HOXA11 antisense RNA) in NSCLC are unknown. HOXA11-AS was knocked down in the NSCLC A549 cell line and a high throughput microarray assay was applied to detect changes in the gene profiles of the A549 cells. Bioinformatics analyses (gene ontology (GO), pathway, Kyoto Encyclopedia of Genes and Genomes (KEGG), and network analyses) were performed to investigate the potential pathways and networks of the differentially expressed genes. The molecular signatures database (MSigDB) was used to display the expression profiles of these differentially expressed genes. Furthermore, the relationships between the HOXA11-AS, de-regulated genes and clinical NSCLC parameters were verified by using NSCLC patient information from The Cancer Genome Atlas (TCGA) database. In addition, the relationship between HOXA11-AS expression and clinical diagnostic value was analyzed by receiver operating characteristic (ROC) curve. Among the differentially expressed genes, 277 and 80 genes were upregulated and downregulated in NSCLC, respectively (fold change ≥2.0, P