Effects of Renal Ischemic Postconditioning on Myocardial Ultrastructural Organization and Myocardial Expression of Bcl-2/Bax in Rabbits

We investigated the cardioprotective effect of renal ischemic postconditioning (RI-PostC) and its mechanisms in a rabbit model. Rabbits underwent 60 min of left anterior descending coronary artery occlusion (LADO) and 6 h of reperfusion. The ischemia-reperfusion (IR) group underwent LADO and reperfu...

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Bibliographic Details
Published in:BioMed research international 2016-01, Vol.2016 (2016), p.1-10
Main Authors: Ning, Xian-feng, Zhang, Jidong, Liu, Song, Li, Rong, Zhang, Wen-zhong, Cai, Shang-lang
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Apoptosis - genetics
bcl-2-Associated X Protein - biosynthesis
bcl-2-Associated X Protein - genetics
Biomedical research
Coronary vessels
Disease Models, Animal
Gene expression
Genetic aspects
Health aspects
Heart
Heart muscle
Humans
Ischemia
Ischemic Postconditioning - methods
Kidney - metabolism
Kidney - pathology
Kinases
Laboratory animals
Myocardial Reperfusion Injury - genetics
Myocardial Reperfusion Injury - physiopathology
Myocardial Reperfusion Injury - therapy
Myocardium - pathology
Myocardium - ultrastructure
Protein Kinase C - genetics
Proto-Oncogene Proteins c-bcl-2 - biosynthesis
Proto-Oncogene Proteins c-bcl-2 - genetics
Rabbits
Rodents
Studies
Veins & arteries
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Summary:We investigated the cardioprotective effect of renal ischemic postconditioning (RI-PostC) and its mechanisms in a rabbit model. Rabbits underwent 60 min of left anterior descending coronary artery occlusion (LADO) and 6 h of reperfusion. The ischemia-reperfusion (IR) group underwent LADO and reperfusion only. In the RI-PostC group, the left renal artery underwent 3 cycles of occlusion for 30 seconds and release for 30 seconds, before the coronary artery was reperfused. In the RI-PostC + GF109203X group, the rabbits received 0.05 mg/kg GF109203X (protein kinase C inhibitor) intravenously for 10 min followed by RI-PostC. Light microscopy and electron microscopy demonstrated that the RI-PostC group showed less pronounced changes, a smaller infarct region, and less apoptosis than the other two groups. Bcl-2 and Bax protein expression did not differ between the IR and RI-PostC + GF109203X groups. However, in the RI-PostC group, Bcl-2 protein expression was significantly higher and Bax protein expression was significantly lower than in the other two groups (P
ISSN:2314-6133
2314-6141
DOI:10.1155/2016/9349437