Gene silencing in alveolar type II cells using cell-specific promoter in vitro and in vivo

RNA interference (RNAi) is a sequence-specific post-transcriptional gene silencing process. Although it is widely used in the loss-of-function studies, none of the current RNAi technologies can achieve cell-specific gene silencing. The lack of cell specificity limits its usage in vivo. Here, we repo...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 2004-01, Vol.32 (17), p.e134-e134
Main Authors: Gou, Deming, Narasaraju, Telugu, Chintagari, Narendranath Reddy, Jin, Nili, Wang, Pengcheng, Liu, Lin
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Animals
Annexin A2 - biosynthesis
Annexin A2 - genetics
Cells, Cultured
Genetic Vectors
Lamins - biosynthesis
Lamins - genetics
Lung - cytology
Lung - metabolism
Male
NAR Methods Online
Organ Culture Techniques
Promoter Regions, Genetic
Pulmonary Alveoli - cytology
Pulmonary Alveoli - metabolism
Pulmonary Surfactant-Associated Protein C - genetics
Rats
Rats, Sprague-Dawley
RNA Interference
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:RNA interference (RNAi) is a sequence-specific post-transcriptional gene silencing process. Although it is widely used in the loss-of-function studies, none of the current RNAi technologies can achieve cell-specific gene silencing. The lack of cell specificity limits its usage in vivo. Here, we report a cell-specific RNAi system using an alveolar epithelial type II cell-specific promoter—the surfactant protein C (SP-C) promoter. We show that the SP-C-driven small hairpin RNAs specifically depress the expression of the exogenous reporter (enhanced green fluorescent protein) and endogenous genes (lamin A/C and annexin A2) in alveolar type II cells, but not other lung cells, using cell and organ culture in vitro as well as in vivo. The present study provides an efficient strategy in silencing a gene in one type of cell without interfering with other cell systems, and may have a significant impact on RNAi therapy.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/gnh129