Altered apoptosis/autophagy and epigenetic modifications cause the impaired postimplantation octaploid embryonic development in mice

Polyploids are pervasive in plants and have large impacts on crop breeding, but natural polyploids are rare in animals. Mouse diploid embryos can be induced to become tetraploid by blastomere fusion at the 2-cell stage and tetraploid embryos can develop to the blastocyst stage in vitro. However, the...

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Bibliographic Details
Published in:Cell cycle (Georgetown, Tex.) Tex.), 2017-01, Vol.16 (1), p.82-90
Main Authors: Wu, Bao-Jiang, Zhao, Li-Xia, Zhu, Cheng-Cheng, Chen, Yang-Lin, Wei, Meng-Yi, Bao, Si-Qin, Sun, Shao-Chen, Li, Xi-He
Format: Article
Language:English
Subjects:
Animals
Apoptosis - genetics
Autophagy - genetics
Biomarkers - metabolism
Blastocyst - cytology
Blastocyst - metabolism
Cell Lineage - genetics
Embryo, Mammalian - cytology
Embryo, Mammalian - metabolism
Embryonic Development - genetics
Epigenesis, Genetic
Female
Mice, Inbred ICR
Models, Biological
Polyploidy
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Summary:Polyploids are pervasive in plants and have large impacts on crop breeding, but natural polyploids are rare in animals. Mouse diploid embryos can be induced to become tetraploid by blastomere fusion at the 2-cell stage and tetraploid embryos can develop to the blastocyst stage in vitro. However, there is little information regarding mouse octaploid embryonic development and precise mechanisms contributing to octaploid embryonic developmental limitations are unknown. To investigate the genetic and epigenetic mechanisms underlying octaploid embryonic development, we generated mouse octaploid embryos and evaluated the in vitro/in vivo developmental potential. Here we show that octaploid embryos can develop to the blastocyst stage in vitro, but all fetus impaired immediately after implantation. Our results indicate that cell lineage specification of octaploid embryo was disorganized. Furthermore, these octaploid embryos showed increased apoptosis as well as alterations in epigenetic modifications when compared with diploid embryos. Thus, our cumulative data provide cues for why mouse octaploid embryonic development is limited and its failed postimplantation development.
ISSN:1538-4101
1551-4005
DOI:10.1080/15384101.2016.1252884