Gut symbiont enhances insecticide resistance in a significant pest, the oriental fruit fly Bactrocera dorsalis (Hendel)

Symbiotic bacteria affect insect physiology and ecology. They may also mediate insecticide resistance within their hosts and thereby impact pest and vector control practices. Here, we document a novel mechanism of insecticide resistance in which a gut symbiont of the tephritid pest fruit fly Bactroc...

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Bibliographic Details
Published in:Microbiome 2017-02, Vol.5 (1), p.13-13, Article 13
Main Authors: Cheng, Daifeng, Guo, Zijun, Riegler, Markus, Xi, Zhiyong, Liang, Guangwen, Xu, Yijuan
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Citrobacter freundii - classification
Citrobacter freundii - isolation & purification
Citrobacter freundii - metabolism
Drug Resistance - physiology
Gastrointestinal Microbiome - drug effects
Gastrointestinal Microbiome - genetics
Gastrointestinal Microbiome - physiology
Hydrolases - genetics
Hydrolases - metabolism
Insecticide Resistance - physiology
Insecticides - pharmacology
RNA, Ribosomal, 16S - genetics
Symbiosis
Tephritidae - drug effects
Tephritidae - microbiology
Trichlorfon - metabolism
Trichlorfon - pharmacology
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Summary:Symbiotic bacteria affect insect physiology and ecology. They may also mediate insecticide resistance within their hosts and thereby impact pest and vector control practices. Here, we document a novel mechanism of insecticide resistance in which a gut symbiont of the tephritid pest fruit fly Bactrocera dorsalis enhances resistance to the organophosphate insecticide trichlorphon. We demonstrated that the gut symbiont Citrobacter sp. (CF-BD) plays a key role in the degradation of trichlorphon. Based on a comparative genomics analysis with other Citrobacter species, phosphatase hydrolase genes were identified in CF-BD. These CF-BD genes had higher expression when trichlorphon was present. Bactrocera dorsalis inoculated with isolated CF-BD obtained higher trichlorphon resistance, while antibiotic-treated flies were less resistant confirming the key role of CF-BD in insecticide resistance. Our findings suggest that symbiont-mediated insecticide resistance can readily develop in B. dorsalis and may represent a more widely relevant insecticide resistance mechanism than previously recognized.
ISSN:2049-2618
2049-2618
DOI:10.1186/s40168-017-0236-z