Tuning the ion selectivity of two-pore channels

Organellar two-pore channels (TPCs) contain two copies of a Shaker-like six-transmembrane (6-TM) domain in each subunit and are ubiquitously expressed in plants and animals. Interestingly, plant and animal TPCs share high sequence similarity in the filter region, yet exhibit drastically different io...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2017-01, Vol.114 (5), p.1009-1014
Main Authors: Guo, Jiangtao, Zeng, Weizhong, Jiang, Youxing
Format: Article
Language:English
Subjects:
Animals
BASIC BIOLOGICAL SCIENCES
Biological Sciences
crystal structure
Flowers & plants
ion selectivity
Ions
Membranes
Mutation
two-pore channel
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Summary:Organellar two-pore channels (TPCs) contain two copies of a Shaker-like six-transmembrane (6-TM) domain in each subunit and are ubiquitously expressed in plants and animals. Interestingly, plant and animal TPCs share high sequence similarity in the filter region, yet exhibit drastically different ion selectivity. Plant TPC1 functions as a nonselective cation channel on the vacuole membrane, whereas mammalian TPC channels have been shown to be endo/lysosomal Na⁺-selective or Ca2+-release channels. In this study, we performed systematic characterization of the ion selectivity of TPC1 from Arabidopsis thaliana (AtTPC1) and compared its selectivity with the selectivity of human TPC2 (HsTPC2). We demonstrate that AtTPC1 is selective for Ca2+ over Na⁺, but nonselective among monovalent cations (Li⁺, Na⁺, and K⁺). Our results also confirm that HsTPC2 is a Na⁺-selective channel activated by phosphatidylinositol 3,5-bisphosphate. Guided by our recent structure of AtTPC1, we converted AtTPC1 to a Na⁺-selective channel by mimicking the selectivity filter of HsTPC2 and identified key residues in the TPC filters that differentiate the selectivity between AtTPC1 and HsTPC2. Furthermore, the structure of the Na⁺-selective AtTPC1 mutant elucidates the structural basis for Na+ selectivity in mammalian TPCs.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1616191114