Serum calreticulin as a novel biomarker of juvenile idiopathic arthritis disease activity

• Published in: European journal of rheumatology 2017-03, Vol.4 (1), p.19-23
• Main Authors: Hashaad, Nashwa Ismail, Fawzy, Rasha Mohamed, Elazem, Abeer Ahmed Abo, Youssef, Mohamed Ibrahim
• Format: Article
• Language: English
• Subjects: Arthritis; Care and treatment; Diagnosis; Endoplasmic reticulum; Enzyme-linked immunosorbent assay; Original Investigation; Ultrasound imaging
• ISSN: 2147-9720; 2148-4279
• DOI: 10.5152/eurjrheum.2017.160071

This study aimed to investigate the relations between calreticulin (CRT) serum level and both disease activity and severity parameters in juvenile idiopathic arthritis (JIA). In this study, 60 children with JIA and 50 age-and-sex-matched healthy subjects were enrolled. The assessment of the disease activity was done using juvenile arthritis disease activity score 27 (JADAS-27). The assessment of disease severity was done via gray-scale ultrasonography (US) and power Doppler US (PDUS). Enzyme-linked immunosorbent assay (ELISA) was used to assay the serum level of human CRT. The mean serum CRT levels in JIA patients was 8.6±1.2 ng/mL and showed a highly significant increase (p=0.001) as compared to the mean serum levels in the controls (5.02±0.77 ng/mL). There were statistically significant positive correlations between the serum CRT levels and disease duration, tender joint count, swollen joint count, visual analog scale, erythrocyte sedimentation rate, JADAS-27, C-reactive protein, rheumatoid factor titer, and ultrasonographic grading for synovitis and neovascularization. Elevated serum CRT levels in JIA patients and its correlations with JIA disease activity and severity parameters signified that CRT might be used as a novel biomarker for disease activity and severity in JIA.