CCL2 nitration is a negative regulator of chemokine-mediated inflammation

Chemokines promote leukocyte recruitment during inflammation. The oxidative burst is an important effector mechanism, this leads to the generation of reactive nitrogen species (RNS), including peroxynitrite (ONOO). The current study was performed to determine the potential for nitration to alter the...

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Bibliographic Details
Published in:Scientific reports 2017-03, Vol.7 (1), p.44384-44384, Article 44384
Main Authors: Barker, Catriona E., Thompson, Sarah, O’Boyle, Graeme, Lortat-Jacob, Hugues, Sheerin, Neil S., Ali, Simi, Kirby, John A.
Format: Article
Language:English
Subjects:
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Animals
Binding Sites
Biochemistry, Molecular Biology
Biological properties
Cell Movement - genetics
Chemokine CCL2 - chemistry
Chemokine CCL2 - metabolism
Chemokines
Chemokines - metabolism
Chemotaxis
Heparan sulfate
Humanities and Social Sciences
Immunofluorescence
Inflammation
Inflammation - metabolism
Inflammation - pathology
Intravenous administration
Kidneys
Leukocyte migration
Leukocytes - metabolism
Life Sciences
Mice
Monocyte chemoattractant protein 1
Monocytes - metabolism
multidisciplinary
Nitration
Nitrogen
Oxidative Stress - genetics
Peroxynitrite
Peroxynitrous Acid - chemistry
Peroxynitrous Acid - metabolism
Reactive nitrogen species
Reactive Nitrogen Species - chemistry
Reactive Nitrogen Species - metabolism
Receptors, CCR2 - genetics
Receptors, CCR2 - metabolism
Science
Spectroscopy
Surface plasmon resonance
Tyrosine
Tyrosine - metabolism
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Summary:Chemokines promote leukocyte recruitment during inflammation. The oxidative burst is an important effector mechanism, this leads to the generation of reactive nitrogen species (RNS), including peroxynitrite (ONOO). The current study was performed to determine the potential for nitration to alter the chemical and biological properties of the prototypical CC chemokine, CCL2. Immunofluorescence was performed to assess the presence of RNS in kidney biopsies. Co-localisation was observed between RNS-modified tyrosine residues and the chemokine CCL2 in diseased kidneys. Nitration reduced the potential of CCL2 to stimulate monocyte migration in diffusion gradient chemotaxis assays (p 
ISSN:2045-2322
2045-2322
DOI:10.1038/srep44384