Differential gene expression associated with inflammation in peripheral blood cells of patients with pneumoconiosis

Objectives To study expression changes in inflammation‐related genes in peripheral blood of patients with pneumoconiosis and to explore the possibility of these genes as pneumoconiosis biomarkers. Methods Peripheral blood samples of patients with pneumoconiosis patients and controls were collected,...

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Bibliographic Details
Published in:Journal of occupational health 2016-07, Vol.58 (4), p.373-380
Main Authors: Liu, Shu‐jun, Wang, Ping, Jiao, Jie, Han, Lin, Lu, Yu‐min
Format: Article
Language:English
Subjects:
Adult
Anthracosis - blood
Anthracosis - etiology
Anthracosis - genetics
Biomarkers
Biomarkers - blood
Blood
Blood cells
Blood Cells - metabolism
Case-Control Studies
Coal Mining
Dust
Female
Gene Expression
Genes
Genetic Markers
Humans
Inflammation
Interleukin-6 - blood
Interleukin-6 - genetics
Male
Middle Aged
Occupational Exposure - adverse effects
Occupational health
Original
Patients
Peripheral blood cells
Pneumoconiosis
Real time
Real-Time Polymerase Chain Reaction
Real‐time quantitative PCR array
RNA, Messenger - blood
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Summary:Objectives To study expression changes in inflammation‐related genes in peripheral blood of patients with pneumoconiosis and to explore the possibility of these genes as pneumoconiosis biomarkers. Methods Peripheral blood samples of patients with pneumoconiosis patients and controls were collected, and total RNA of the blood cells were extracted and reverse transcribed to cDNA. Screenings of deferentially expressed genes associated with inflammation between patients with pneumoconiosis and controls were performed using real‐time quantitative PCR array and the expressions of the three most upregulated genes were confirmed by real‐time PCR. Results The expression of 11 genes was significantly altered in patients with pneumoconiosis compared with those of the control. Among these 11 genes, 8 genes were upregulated and 3 were downregulated. Preliminary results indicated that interleukin 6 (IL‐6) mRNA expression in patients with pneumoconiosis was higher than that in controls (P=0.019). The level of IL6 mRNA expression in the patients was higher than that in non‐smoking controls, but it was neither affected by type and stage of pneumoconiosis nor by time of contact with dust. Conclusions IL6 was possibly involved in the development of pneumoconiosis.
ISSN:1348-9585
1341-9145
1348-9585
DOI:10.1539/joh.16-0021-OA