Laser beam melting 3D printing of Ti6Al4V based porous structured dental implants: fabrication, biocompatibility analysis and photoelastic study

• Published in: Scientific reports 2017-03, Vol.7 (1), p.45360-45360, Article 45360
• Main Authors: Yang, Fei, Chen, Chen, Zhou, QianRong, Gong, YiMing, Li, RuiXue, Li, ChiChi, Klämpfl, Florian, Freund, Sebastian, Wu, XingWen, Sun, Yang, Li, Xiang, Schmidt, Michael, Ma, Duan, Yu, YouCheng
• Format: Article
• Language: English
• Subjects: 14/28; 3-D printers; 38/39; 639/301/54/993; 639/624/1111/1116; Alkaline Phosphatase - genetics; Alkaline Phosphatase - metabolism; Animals; Biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Bone implants; Cell growth; Cell Line; Cell proliferation; Cell Survival - drug effects; Dental Implants; Dental prosthetics; Dental restorative materials; Elasticity; Fabrication; Humanities and Social Sciences; Immunofluorescence; Lasers; Mathematical models; Melting; Mice; multidisciplinary; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - metabolism; Osteogenesis - drug effects; Pore size; Porosity; Printing; Printing, Three-Dimensional; Science; Titanium - chemistry; Transplants & implants
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep45360

Fabricating Ti alloy based dental implants with defined porous scaffold structure is a promising strategy for improving the osteoinduction of implants. In this study, we use Laser Beam Melting (LBM) 3D printing technique to fabricate porous Ti6Al4V dental implant prototypes with three controlled pore sizes (200, 350 and 500 μm). The mechanical stress distribution in the surrounding bone tissue is characterized by photoelastography and associated finite element simulation. For in-vitro studies, experiments on implants’ biocompatibility and osteogenic capability are conducted to evaluate the cellular response correlated to the porous structure. As the preliminary results, porous structured implants show a lower stress-shielding to the surrounding bone at the implant neck and a more densed distribution at the bottom site compared to the reference implant. From the cell proliferation tests and the immunofluorescence images, 350 and 500 μm pore sized implants demonstrate a better biocompatibility in terms of cell growth, migration and adhesion. Osteogenic genes expression of the 350 μm group is significantly increased alone with the ALP activity test. All these suggest that a pore size of 350 μm provides an optimal provides an optimal potential for improving the mechanical shielding to the surrounding bones and osteoinduction of the implant itself.