Investigation of ac-magnetic field stimulated nanoelectroporation of magneto-electric nano-drug-carrier inside CNS cells

In this research, we demonstrate cell uptake of magneto-electric nanoparticles (MENPs) through nanoelectroporation (NEP) using alternating current (ac)-magnetic field stimulation. Uptake of MENPs was confirmed using focused-ion-beam assisted transmission electron microscopy (FIB-TEM) and validated b...

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Bibliographic Details
Published in:Scientific reports 2017-04, Vol.7 (1), p.45663-45663, Article 45663
Main Authors: Kaushik, Ajeet, Nikkhah-Moshaie, Roozbeh, Sinha, Raju, Bhardwaj, Vinay, Atluri, Venkata, Jayant, Rahul Dev, Yndart, Adriana, Kateb, Babak, Pala, Nezih, Nair, Madhavan
Format: Article
Language:English
Subjects:
13
14
631/1647/350
692/308
Brain - drug effects
Cell Line
Central nervous system
Drug Carriers
Electricity
Electron microscopy
Electroporation - methods
Humanities and Social Sciences
Humans
Magnetic Fields
Mathematical models
Microglia - drug effects
Microscopy, Electron, Transmission - methods
multidisciplinary
Nanomedicine - methods
Nanoparticles
Nanoparticles - chemistry
Nanotechnology
Science
Transmission electron microscopy
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Summary:In this research, we demonstrate cell uptake of magneto-electric nanoparticles (MENPs) through nanoelectroporation (NEP) using alternating current (ac)-magnetic field stimulation. Uptake of MENPs was confirmed using focused-ion-beam assisted transmission electron microscopy (FIB-TEM) and validated by a numerical simulation model. The NEP was performed in microglial (MG) brain cells, which are highly sensitive for neuro-viral infection and were selected as target for nano-neuro-therapeutics. When the ac-magnetic field optimized (60 Oe at 1 kHz), MENPs were taken up by MG cells without affecting cell health (viability > 92%). FIB-TEM analysis of porated MG cells confirmed the non-agglomerated distribution of MENPs inside the cell and no loss of their elemental and crystalline characteristics. The presented NEP method can be adopted as a part of future nanotherapeutics and nanoneurosurgery strategies where a high uptake of a nanomedicine is required for effective and timely treatment of brain diseases.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep45663