Controllable Activation of Nanoscale Dynamics in a Disordered Protein Alters Binding Kinetics

• Published in: Journal of molecular biology 2017-04, Vol.429 (7), p.987-998
• Main Authors: Callaway, David J.E., Matsui, Tsutomu, Weiss, Thomas, Stingaciu, Laura R., Stanley, Christopher B., Heller, William T., Bu, Zimei
• Format: Article
• Language: English
• Subjects: BASIC BIOLOGICAL SCIENCES; Chromatography, Gel; disordered protein; Humans; Kinetics; Models, Molecular; Mutant Proteins - chemistry; Mutant Proteins - genetics; Mutant Proteins - metabolism; nanoscale protein motion; neutron spin echo spectroscopy; Phosphoproteins - chemistry; Phosphoproteins - genetics; Phosphoproteins - metabolism; Phosphorylation; Protein Binding; protein binding kinetics; Protein Conformation; protein dynamics; Protein Processing, Post-Translational; Scattering, Small Angle; Sodium-Hydrogen Exchangers - chemistry; Sodium-Hydrogen Exchangers - genetics; Sodium-Hydrogen Exchangers - metabolism; Spectrum Analysis
• ISSN: 0022-2836; 1089-8638
• DOI: 10.1016/j.jmb.2017.03.003

The phosphorylation of specific residues in a flexible disordered activation loop yields precise control of signal transduction. One paradigm is the phosphorylation of S339/S340 in the intrinsically disordered tail of the multi-domain scaffolding protein NHERF1, which affects the intracellular localization and trafficking of NHERF1 assembled signaling complexes. Using neutron spin echo spectroscopy (NSE), we show salt-concentration-dependent excitation of nanoscale motion at the tip of the C-terminal tail in the phosphomimic S339D/S340D mutant. The “tip of the whip” that is unleashed is near the S339/S340 phosphorylation site and flanks the hydrophobic Ezrin-binding motif. The kinetic association rate constant of the binding of the S339D/S340D mutant to the FERM domain of Ezrin is sensitive to buffer salt concentration, correlating with the excited nanoscale dynamics. The results suggest that electrostatics modulates the activation of nanoscale dynamics of an intrinsically disordered protein, controlling the binding kinetics of signaling partners. NSE can pinpoint the nanoscale dynamics changes in a highly specific manner. [Display omitted] •Nanoscale protein dynamics in an intrinsically disordered protein affects protein–protein interactions in a largely unknown fashion because it is experimentally difficult to probe protein dynamics on nanoscales.•By employing the novel technique of NSE, we are able to reveal nanoscale dynamics changes in a disordered protein.•Our theoretical analysis shows that NSE pinpoints the nanoscale dynamics changes in a highly specific manner.•Debye–Hückel analysis indicates that changes in kinetic association rate constant at different ionic strengths cannot be explained by electrostatic effects alone.•This study shows that nanoscale dynamics in a disordered protein significantly influences the binding kinetics of signaling partners.