EphB1 and EphB2 intracellular domains regulate the formation of the corpus callosum and anterior commissure

ABSTRACT The two cortical hemispheres of the mammalian forebrain are interconnected by major white matter tracts, including the corpus callosum (CC) and the posterior branch of the anterior commissure (ACp), that bridge the telencephalic midline. We show here that the intracellular signaling domains...

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Published in:Developmental neurobiology (Hoboken, N.J.) N.J.), 2016-04, Vol.76 (4), p.405-420
Main Authors: Robichaux, Michael A., Chenaux, George, Ho, Hsin‐Yi Henry, Soskis, Michael J., Greenberg, Michael E., Henkemeyer, Mark, Cowan, Christopher W.
Format: Article
Language:English
Subjects:
Animals
Anterior Cerebellar Commissure - cytology
Anterior Cerebellar Commissure - embryology
Anterior Cerebellar Commissure - metabolism
anterior commissure
axon guidance
Axons - metabolism
Cell Movement - physiology
corpus callosum
Corpus Callosum - cytology
Corpus Callosum - embryology
Corpus Callosum - metabolism
EphB
ephrin
Gene Knock-In Techniques
Gene Knockout Techniques
Immunohistochemistry
Intracellular Space
Mice, Transgenic
Neuroanatomical Tract-Tracing Techniques
Protein Domains
Receptor, EphB1 - genetics
Receptor, EphB1 - metabolism
Receptor, EphB2 - genetics
Receptor, EphB2 - metabolism
Signal Transduction
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Summary:ABSTRACT The two cortical hemispheres of the mammalian forebrain are interconnected by major white matter tracts, including the corpus callosum (CC) and the posterior branch of the anterior commissure (ACp), that bridge the telencephalic midline. We show here that the intracellular signaling domains of the EphB1 and EphB2 receptors are critical for formation of both the ACp and CC. We observe partial and complete agenesis of the corpus callosum, as well as highly penetrant ACp misprojection phenotypes in truncated EphB1/2 mice that lack intracellular signaling domains. Consistent with the roles for these receptors in formation of the CC and ACp, we detect expression of these receptors in multiple brain regions associated with the formation of these forebrain structures. Taken together, our findings suggest that a combination of forward and reverse EphB1/2 receptor‐mediated signaling contribute to ACp and CC axon guidance. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 405–420, 2016
ISSN:1932-8451
1932-846X
DOI:10.1002/dneu.22323