Mutual transrepression of Fos and the glucocorticoid receptor: involvement of a functional domain in Fos which is absent in FosB

In this study, we show that Fos protein can repress transactivation by the glucocorticoid receptor (GR). In addition, we demonstrate that GR is capable of inhibiting, in a hormone‐dependent fashion, Fos‐mediated transactivation of AP‐1 dependent transcription. Moreover, repression of the serum respo...

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Bibliographic Details
Published in:The EMBO journal 1990-09, Vol.9 (9), p.2827-2834
Main Authors: Lucibello, F. C., Slater, E. P., Jooss, K. U., Beato, M., Müller, R.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Cell Line
DNA-Binding Proteins - metabolism
Enzyme Repression
Fundamental and applied biological sciences. Psychology
HeLa Cells - metabolism
Humans
Mice
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Mutation
Oligonucleotide Probes
Plasmids
Promoter Regions, Genetic
Protein-Tyrosine Kinases - genetics
Proto-Oncogene Proteins - biosynthesis
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins c-fos
Receptors, Glucocorticoid - biosynthesis
Receptors, Glucocorticoid - genetics
Receptors, Glucocorticoid - metabolism
Restriction Mapping
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
Transfection
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Summary:In this study, we show that Fos protein can repress transactivation by the glucocorticoid receptor (GR). In addition, we demonstrate that GR is capable of inhibiting, in a hormone‐dependent fashion, Fos‐mediated transactivation of AP‐1 dependent transcription. Moreover, repression of the serum response element by Fos is abolished by the GR in the presence of hormone. Transrepression of glucocorticoid mediated induction involves a region of Fos, located between amino acids 40 and 111, to which no function has been previously assigned, and which is poorly conserved among Fos, FosB and Fra‐1. In agreement with this finding, FosB is not capable of transrepressing GR activation of transcription, representing the first functional difference between Fos and FosB. We have mapped the domain of the GR which is required for repression of AP‐1 dependent transcription, to the region of central DNA binding domain. Our results suggest that Fos and the GR may form transcriptionally inactive complexes and point to a regulatory interrelationship between different signal transduction pathways.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1990.tb07471.x