Translating next generation sequencing to practice: Opportunities and necessary steps

Next-generation sequencing (NGS) approaches for measuring RNA and DNA benefit from greatly increased sensitivity, dynamic range and detection of novel transcripts. These technologies are rapidly becoming the standard for molecular assays and represent huge potential value to the practice of oncology...

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Bibliographic Details
Published in:Molecular oncology 2013-08, Vol.7 (4), p.743-755
Main Authors: Kamalakaran, Sitharthan, Varadan, Vinay, Janevski, Angel, Banerjee, Nilanjana, Tuck, David, McCombie, W. Richard, Dimitrova, Nevenka, Harris, Lyndsay N.
Format: Article
Language:English
Subjects:
Adoption
Algorithms
Breast cancer
Cancer therapies
Codes
Copy number
DNA Copy Number Variations - genetics
DNA sequencing
Gene expression
Genomes
Genomics
Genomics - methods
High-Throughput Nucleotide Sequencing - methods
Humans
Male
Mutation
Neoplasms - genetics
Next generation sequencing
Oncology
Pathology
Patients
Personalized medicine
Review
Ribonucleic acid
RNA
Sequence Analysis, DNA
Surgery
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Summary:Next-generation sequencing (NGS) approaches for measuring RNA and DNA benefit from greatly increased sensitivity, dynamic range and detection of novel transcripts. These technologies are rapidly becoming the standard for molecular assays and represent huge potential value to the practice of oncology. However, many challenges exist in the transition of these technologies from research application to clinical practice. This review discusses the value of NGS in detecting mutations, copy number changes and RNA quantification and their applications in oncology, the challenges for adoption and the relevant steps that are needed for translating this potential to routine practice. •Next Generation sequencing (NGS) enables measurement of clinically relevant mutations, DNA copy number and gene expression.•We review diagnostic, prognostic and therapy selection applications of NGS for different types of cancer.•We discuss technology challenges that need to be overcome for implementing NGS into widespread clinical use.•We discuss education, regulatory framework, storage, privacy and confidentiality of genomic data to enable adoption.
ISSN:1574-7891
1878-0261
DOI:10.1016/j.molonc.2013.04.008