Human Mesenchymal Stem Cells in Rodent Whole-Embryo Culture Are Reprogrammed to Contribute to Kidney Tissues

The use of stem cells has enabled the successful generation of simple organs. However, anatomically complicated organs such as the kidney have proven more refractory to stem-cell-based regenerative techniques. Given the limits of allogenic organ transplantation, an ultimate therapeutic solution is t...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2005-03, Vol.102 (9), p.3296-3300
Main Authors: Yokoo, Takashi, Ohashi, Toya, Shen, Jin Song, Sakurai, Ken, Miyazaki, Yoichi, Utsunomiya, Yasunori, Takahashi, Masanori, Terada, Yoshio, Eto, Yoshikatsu, Kawamura, Tetsuya, Osumi, Noriko, Hosoya, Tatsuo, Ruoslahti, Erkki
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Cellular biology
Coculture Techniques
Cultured cells
Embryo, Mammalian - cytology
Embryos
Epithelial cells
Human organs
Humans
Interstitial cells
Kidney - cytology
Kidney cells
Kidneys
Mesenchymal stem cells
Mesoderm - cytology
Neuroglia
Organ culture techniques
Rats
Rats, Sprague-Dawley
Rodents
Stem cells
Stem Cells - cytology
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Summary:The use of stem cells has enabled the successful generation of simple organs. However, anatomically complicated organs such as the kidney have proven more refractory to stem-cell-based regenerative techniques. Given the limits of allogenic organ transplantation, an ultimate therapeutic solution is to establish self-organs from autologous stem cells and transplant them as syngrafts back into donor patients. To this end, we have striven to establish an in vitro organ factory to build up complex organ structures from autologous adult stem cells by using the kidney as a target organ. Cultivation of human mesenchymal stem cells in growing rodent embryos enables their differentiation within a spatially and temporally appropriate developmental milieu, facilitating the first step of nephrogenesis. We show that a combination of whole-embryo culture, followed by organ culture, encourages exogenous human mesenchymal stem cells to differentiate and contribute to functional complex structures of the new kidney.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0406878102