Peri‐prosthetic tissue cells show osteogenic capacity to differentiate into the osteoblastic lineage

ABSTRACT During the process of aseptic loosening of prostheses, particulate wear debris induces a continuous inflammatory‐like response resulting in the formation of a layer of fibrous peri‐prosthetic tissue at the bone‐prosthesis interface. The current treatment for loosening is revision surgery wh...

Full description

Saved in:
Bibliographic Details
Published in:Journal of orthopaedic research 2017-08, Vol.35 (8), p.1732-1742
Main Authors: Schoeman, Monique A.E., Oostlander, Angela E., Rooij, Karien Ede, Valstar, Edward R., Nelissen, Rob G.H.H.
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Aseptic loosening
Cell Differentiation
Female
Hip Prosthesis
Humans
Male
Middle Aged
Osseointegration
Osteoblasts
osteogenic differentiation
peri‐prosthetic tissue
Primary Cell Culture
Prosthesis Failure
regenerative medicine
total hip revision
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:ABSTRACT During the process of aseptic loosening of prostheses, particulate wear debris induces a continuous inflammatory‐like response resulting in the formation of a layer of fibrous peri‐prosthetic tissue at the bone‐prosthesis interface. The current treatment for loosening is revision surgery which is associated with a high‐morbidity rate, especially in old patients. Therefore, less invasive alternatives are necessary. One approach could be to re‐establish osseointegration of the prosthesis by inducing osteoblast differentiation in the peri‐prosthetic tissue. Therefore, the aim of this study was to investigate the capacity of peri‐prosthetic tissue cells to differentiate into the osteoblast lineage. Cells isolated from peri‐prosthetic tissue samples (n = 22)−obtained during revision surgeries−were cultured under normal and several osteogenic culture conditions. Osteogenic differentiation was assessed by measurement of Alkaline Phosphatse (ALP), mineralization of the matrix and expression of several osteogenic genes. Cells cultured in osteogenic medium showed a significant increase in ALP staining (p = 0.024), mineralization of the matrix (p 
ISSN:0736-0266
1554-527X
DOI:10.1002/jor.23457